Expression of cutaneous lymphocyte-associated antigen by CD8+ T cells specific for a skin-tropic virus Journal Article
| Authors: | Koelle, D. M.; Liu, Z.; McClurkan, C. M.; Topp, M. S.; Riddell, S. R.; Pamer, E. G.; Johnson, A. S.; Wald, A.; Corey, L. |
| Article Title: | Expression of cutaneous lymphocyte-associated antigen by CD8+ T cells specific for a skin-tropic virus |
| Abstract: | Virus-specific CD8+ T cells traffic to infected tissues to promote clearance of infection. We used herpes simplex virus type 2 (HSV-2) as a model system to investigate CD8+ T cell trafficking to the skin in humans. Using human leukocyte antigen (HLA) class I tetramers, we observed that HSV-specific CD8+ T cells in the peripheral blood expressed high levels of cutaneous lymphocyte-associated antigen (CLA). In contrast, CD8+ T cells specific for non-skin-tropic herpesviruses lacked CLA expression. CLA-positive HSV-2-specific CD8+ T cells had the characteristics of central memory cells, expressing CCR7, CD62L, and CD28, and they proliferated briskly in response to antigen. CLA is related to a functional E-selectin ligand, and both E-selectin and CLA-positive cells were detected in HSV-2-infected skin. HSV-2-specific T cells adhered to cells transfected with E-selectin. A higher proportion of HSV-specific CD8+ T cells recovered from herpes lesions express CLA compared with blood, consistent with a role for CLA in skin homing. To our knowledge, this is the first report of expression of tissue-specific adhesion-associated molecules by virus-specific CD8+ T cells. The evaluation of vaccines for skin and mucosal pathogens should include study of the induction of appropriate tissue-specific homing molecules. |
| Keywords: | controlled study; protein expression; unclassified drug; nonhuman; cd8 antigen; antigen expression; cell proliferation; t lymphocyte; cd8-positive t-lymphocytes; animal cell; animals; cells, cultured; skin; lymphocyte activation; genetic transfection; antigens, neoplasm; peripheral blood stem cell; membrane glycoproteins; hla antigen class 1; cell movement; cell line, transformed; ligands; immunophenotyping; memory cell; chemokine receptor ccr7; herpes; cd28 antigen; immunologic memory; skin infection; endothelial leukocyte adhesion molecule 1; cell adhesion molecule; tissue specificity; cho cells; cricetinae; padgem protein; herpes simplex virus 2; lymphocyte antigen; virus vaccine; herpes genitalis; humans; human; priority journal; article; cutaneous lymphocyte associated antigen; herpesvirus 2, human |
| Journal Title: | Journal of Clinical Investigation |
| Volume: | 110 |
| Issue: | 4 |
| ISSN: | 0021-9738 |
| Publisher: | American Society for Clinical Investigation |
| Date Published: | 2002-08-15 |
| Start Page: | 537 |
| End Page: | 548 |
| Language: | English |
| DOI: | 10.1172/jci200215537 |
| PUBMED: | 12189248 |
| PROVIDER: | scopus |
| PMCID: | PMC150419 |
| DOI/URL: | |
| Notes: | Export Date: 14 November 2014 -- Source: Scopus |
