CT7 (MAGE-C1) antigen expression in normal and neoplastic tissues Journal Article
| Authors: | Jungbluth, A. A.; Chen, Y. T.; Busam, K. J.; Coplan, K.; Kolb, D.; Iversen, K.; Williamson, B.; Van Landeghem, F. K. H.; Stockert, E.; Old, L. J. |
| Article Title: | CT7 (MAGE-C1) antigen expression in normal and neoplastic tissues |
| Abstract: | CT7 (MAGE-C1) is a member of the cancer testis (CT) antigen family. The present study describes the generation of CT7-33, a monoclonal antibody (MAb) to CT7, and the preliminary protein expression analysis of CT7 in normal tissues and in a limited number of neoplastic lesions. CT7-33 was effective in frozen as well as formalin-fixed, paraffin-embedded tissues, and immunohistochemistry/reverse transcriptase polymerase chain reaction (RT-PCR) co-typing demonstrated antibody specificity. CT7-33 immunoreactivity in normal adult tissues is restricted to testicular germ cells. In neoplastic lesions, CT7-33 immunostaining is confined to tumor cells, and the frequency of CT7 protein expression mostly parallels previous mRNA analyses. Whereas colorectal and renal cell carcinomas, as well as sarcomas, exhibit poor or no CT7-33 staining, carcinomas of the mammary gland and ovary, nonsmall cell lung carcinoma and metastatic melanomas exhibit a high incidence of CT7 protein expression. However, as seen in previous analyses of other CT antigens, the expression pattern is mostly heterogeneous, and tumors with more than 50% of tumor staining are only infrequently encountered. In summary, our study presents a new serologic reagent for the analysis of CT7 on a protein level and confirms what is known with regard to the expression pattern of other CT antigens in tumors: frequent heterogeneity of antigen expression. © 2002 Wiley-Liss, Inc. |
| Keywords: | immunohistochemistry; controlled study; human tissue; protein expression; unclassified drug; nonhuman; neoplasm; neoplasms; antigen expression; animal cell; mouse; animals; mice; reverse transcription polymerase chain reaction; gene expression; germ cell; lung non small cell cancer; incidence; neoplasm proteins; animal experiment; immunoreactivity; tumor antigen; mice, inbred balb c; kidney carcinoma; monoclonal antibody; colorectal carcinoma; sarcoma; immunoenzyme techniques; antibodies, monoclonal; antigens, neoplasm; messenger rna; reverse transcriptase polymerase chain reaction; rna, messenger; cancer testis antigen; breast carcinoma; recombinant proteins; tumor cell; ovary carcinoma; antibody specificity; dna primers; protein family; serology; paraffin; immunization; paraffin embedding; mage-c1; rna analysis; humans; human; priority journal; article; ct7; heterogeneous antigen expression; monoclonal antibody ct7-33 |
| Journal Title: | International Journal of Cancer |
| Volume: | 99 |
| Issue: | 6 |
| ISSN: | 0020-7136 |
| Publisher: | John Wiley & Sons |
| Date Published: | 2002-06-20 |
| Start Page: | 839 |
| End Page: | 845 |
| Language: | English |
| DOI: | 10.1002/ijc.10416 |
| PUBMED: | 12115486 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 14 November 2014 -- Source: Scopus |
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