In vivo depletion of CD11c+ dendritic cells abrogates priming of CD8+ T cells by exogenous cell-associated antigens Journal Article
| Authors: | Jung, S.; Unutmaz, D.; Wong, P.; Sano, G. I.; De Los Santos, K.; Sparwasser, T.; Wu, S.; Vuthoori, S.; Ko, K.; Zavala, F.; Pamer, E. G.; Littman, D. R.; Lang, R. A. |
| Article Title: | In vivo depletion of CD11c+ dendritic cells abrogates priming of CD8+ T cells by exogenous cell-associated antigens |
| Abstract: | Cytotoxic T lymphocytes (CTL) respond to antigenic peptides presented on MHC class I molecules. On most cells, these peptides are exclusively of endogenous, cytosolic origin. Bone marrow-derived antigen-presenting cells, however, harbor a unique pathway for MHC I presentation of exogenous antigens. This mechanism permits cross-presentation of pathogen-infected cells and the priming of CTL responses against intracellular microbial infections. Here, we report a novel diphtheria toxin-based system that allows the inducible, short-term ablation of dendritic cells (DC) in vivo. We show that in vivo DC are required to cross-prime CTL precursors. Our results thus define a unique in vivo role of DC, i.e., the sensitization of the immune system for cell-associated antigens. DC-depleted mice fail to mount CTL responses to infection with the intracellular bacterium Listeria monocytogenes and the rodent malaria parasite Plasmodium yoelii. |
| Keywords: | controlled study; nonhuman; t lymphocyte; cd8-positive t-lymphocytes; animal cell; mouse; animals; mice; immune system; dendritic cell; animal experiment; animal model; in vivo study; mice, inbred balb c; mice, inbred c57bl; mice, transgenic; models, animal; liver; dendritic cells; immune response; recombinant fusion proteins; nucleotide sequence; cytotoxic t lymphocyte; t-lymphocytes, cytotoxic; listeriosis; listeria monocytogenes; diphtheria toxin; sensitization; receptors, cell surface; malaria; t lymphocyte antigen; glycoprotein p 15095; priority journal; article; plasmodium yoelii; integrin alphaxbeta2 |
| Journal Title: | Immunity |
| Volume: | 17 |
| Issue: | 2 |
| ISSN: | 1074-7613 |
| Publisher: | Cell Press |
| Date Published: | 2002-08-01 |
| Start Page: | 211 |
| End Page: | 220 |
| Language: | English |
| DOI: | 10.1016/s1074-7613(02)00365-5 |
| PUBMED: | 12196292 |
| PROVIDER: | scopus |
| PMCID: | PMC3689299 |
| DOI/URL: | |
| Notes: | Export Date: 14 November 2014 -- Source: Scopus |
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