Characterization of the mRNA capping apparatus of the microsporidian parasite Encephalitozoon cuniculi Journal Article
| Authors: | Hausmann, S.; Vivarès, C. P.; Shuman, S. |
| Article Title: | Characterization of the mRNA capping apparatus of the microsporidian parasite Encephalitozoon cuniculi |
| Abstract: | A scheme of eukaryotic phylogeny has been suggested based on the structure and physical linkage of the enzymes that catalyze mRNA cap formation. Here we show that the intracellular parasite Encephalitozoon cuniculi encodes a complete mRNA capping apparatus consisting of separate triphosphatase (EcCet1), guanylyltransferase (EcCeg1), and methyltransferase (Ecm1) enzymes, which we characterize biochemically and genetically. The triphosphatase EcCet1 belongs to a metal-dependent phosphohydrolase family that includes the triphosphatase components of the capping apparatus of fungi, DNA viruses, and the malaria parasite Plasmodium falciparum. These enzymes are structurally and mechanistically unrelated to the metal-independent cysteine phosphatase-type RNA triphosphatases found in metazoans and plants. Our findings support the proposed evolutionary connection between microsporidia and fungi, and they place fungi and protozoa in a common lineage distinct from that of metazoans and plants. RNA triphosphatase presents an attractive target for antiprotozoal/antifungal drug development. |
| Keywords: | controlled study; nonhuman; drug targeting; animals; phosphatase; antifungal agent; antiprotozoal agent; acid anhydride hydrolases; structure-activity relationship; rna; methyltransferase; dna; molecular evolution; amino acid sequence; molecular sequence data; messenger rna; dna viruses; eukaryota; rna viruses; catalysis; plasmodium falciparum; genetic code; protozoa; fungus; fungi; enzymes; enzyme mechanism; multigene family; metazoa; phylogeny; characterization; malaria; rna capping; nucleotidyltransferases; viruses; dna virus; parasite; metazoon; microsporida; encephalitozoon cuniculi; microsporidia; priority journal; article; eukaryotic phylogeny; protozoal dna |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 277 |
| Issue: | 1 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 2002-01-04 |
| Start Page: | 96 |
| End Page: | 103 |
| Language: | English |
| DOI: | 10.1074/jbc.M109649200 |
| PUBMED: | 11687593 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 14 November 2014 -- Source: Scopus |
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