High-affinity mu opioid receptor ligands discovered by the screening of an exhaustively stereodiversified library of 1,5-enediols Journal Article
| Authors: | Harrison, B. A.; Gierasch, T. M.; Neilan, C.; Pasternak, G. W.; Verdine, G. L. |
| Article Title: | High-affinity mu opioid receptor ligands discovered by the screening of an exhaustively stereodiversified library of 1,5-enediols |
| Abstract: | In this communication, we report the synthesis of an exhaustively stereodiversified library of 16 1,5-enediols (2) and the screening of these compounds for mu opioid receptor (MOR) binding. The stereochemical configuration of 2 strongly impacted the binding affinity, and (S,S,S,R)-2 exhibited a Ki of 8.8 nM for MOR, comparable to that of endomorphin-2 (Ki = 1.2 nM). Moreover, compounds 2 exhibited 5-86-fold selectivity for MOR over delta opioid receptor (DOR) and 16-150-fold selectivity for MOR over kappa opioid receptor (KOR). Additionally, analogues of 2 were synthesized which showed the importance of the trans olefin for receptor binding but that modifications of the C-terminal amino acid were well tolerated. Ligand 11 is noteworthy because it retains only one of the amide bonds present in 1, but binds MOR with an affinity of 10 nM and 110- and 600-fold selectivity for MOR over DOR and KOR. These results demonstrate the utility of stereochemical diversity in the discovery of bioactive small molecules. Copyright © 2002 American Chemical Society. |
| Keywords: | unclassified drug; binding affinity; kinetics; screening; substrate specificity; ligand; ligands; ligand binding; mu opiate receptor; receptors, opioid, mu; receptor binding; binding competition; stereochemistry; stereoisomerism; combinatorial chemistry techniques; dipeptides; receptors, opioid, delta; alkene derivative; alkenes; alcohols; article; receptors, opioid, kappa; 1,5 enediol |
| Journal Title: | Journal of the American Chemical Society |
| Volume: | 124 |
| Issue: | 45 |
| ISSN: | 0002-7863 |
| Publisher: | American Chemical Society |
| Date Published: | 2002-11-13 |
| Start Page: | 13352 |
| End Page: | 13353 |
| Language: | English |
| DOI: | 10.1021/ja027150p |
| PUBMED: | 12418865 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 14 November 2014 -- Source: Scopus |
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