Plzf mediates transcriptional repression of HoxD gene expression through chromatin remodeling Journal Article
| Authors: | Barna, M.; Merghoub, T.; Costoya, J. A.; Ruggero, D.; Branford, M.; Bergia, A.; Samori, B.; Pandolfi, P. P. |
| Article Title: | Plzf mediates transcriptional repression of HoxD gene expression through chromatin remodeling |
| Abstract: | The molecular mechanism that regulate coordinated and colinear activation of Hox gene expression in space and time remain poorly understood. Here we demonstrate that Plzf regulates the spatial expression of the AbdB HoxD gene complex by binding to regulatory elements required for restricted Hox gene expression and can recruit histone deacetylases to these sites. We show by scanning forced microscopy that Plzf, via homodimerization, can form DNA loops and bridge distant Plzf binding sites located within Hoxd gene regulatory elements. Furthermore, we demonstrate that Plzf physically interacts with Polycomb proteins on DNA. We propose a model by which the balance between activating morphogenic siganls and transcriptional repressors such as Plzf establishes proper Hox gene expression boundaries in the limb bud. |
| Keywords: | signal transduction; controlled study; dna-binding proteins; nonhuman; animal cell; mouse; animals; mice; nuclear protein; embryo; protein protein interaction; morphogenesis; homeodomain proteins; transcription, genetic; time; animalia; transcription factors; gene expression regulation; gene expression regulation, developmental; dna; transcription regulation; chromatin; gene repression; binding site; dimerization; repressor proteins; histones; histone deacetylase; hox protein; limb development; limb bud; microscopy, atomic force; spatial discrimination; article; scanning force microscopy |
| Journal Title: | Developmental Cell |
| Volume: | 3 |
| Issue: | 4 |
| ISSN: | 1534-5807 |
| Publisher: | Cell Press |
| Date Published: | 2002-10-01 |
| Start Page: | 499 |
| End Page: | 510 |
| Language: | English |
| DOI: | 10.1016/s1534-5807(02)00289-7 |
| PUBMED: | 12408802 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 14 November 2014 -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work