Adenylation of maternally inherited MicroRNAs by Wispy Journal Article

Authors: Lee, M.; Choi, Y.; Kim, K.; Jin, H.; Lim, J.; Nguyen, T. A.; Yang, J.; Jeong, M.; Giraldez, A. J.; Yang, H.; Patel, D. J.; Kim, V. N.
Article Title: Adenylation of maternally inherited MicroRNAs by Wispy
Abstract: Early development depends heavily on accurate control of maternally inherited mRNAs, and yet it remains unknown how maternal microRNAs are regulated during maternal-to-zygotic transition (MZT). We here find that maternal microRNAs are highly adenylated at their 3' ends in mature oocytes and early embryos. Maternal microRNA adenylation is widely conserved in fly, sea urchin, and mouse. We identify Wispy, a noncanonical poly(A) polymerase, as the enzyme responsible for microRNA adenylation in flies. Knockout of wispy abrogates adenylation and results in microRNA accumulation in eggs, whereas overexpression of Wispy increases adenylation and reduces microRNA levels in S2 cells. Wispy interacts with Ago1 through protein-protein interaction, which may allow the effective and selective adenylation of microRNAs. Thus, adenylation may contribute to the clearance of maternally deposited microRNAs during MZT. Our work provides mechanistic insights into the regulation of maternal microRNAs and illustrates the importance of RNA tailing in development.
Keywords: echinoidea
Journal Title: Molecular Cell
Volume: 56
Issue: 5
ISSN: 1097-2765
Publisher: Cell Press  
Date Published: 2014-12-04
Start Page: 696
End Page: 707
Language: English
DOI: 10.1016/j.molcel.2014.10.011
PROVIDER: scopus
PUBMED: 25454948
PMCID: PMC4378961
Notes: Export Date: 2 January 2015 -- Source: Scopus
Citation Impact
MSK Authors
  1. Dinshaw J Patel
    457 Patel
  2. Hui-Ju Yang
    1 Yang