Spatiotemporal dynamics of triglyceride storage in unilocular adipocytes Journal Article


Authors: Chu, M.; Sampath, H.; Cahana, D. Y.; Kahl, C. A.; Somwar, R.; Cornea, A.; Roberts, C. T.; Varlamov, O.
Article Title: Spatiotemporal dynamics of triglyceride storage in unilocular adipocytes
Abstract: The spatiotemporal dynamics of triglyceride (TG) storage in unilocular adipocytes are not well understood. Here we applied ex vivo technology to study trafficking and metabolism of fluorescent fatty acids in adipose tissue explants. Live imaging revealed mul? tiple cytoplasmic nodules surrounding the large central lipid droplet (cLD) of unilocular adi? pocytes. Each cytoplasmic nodule harbors a series of closely associated cellular organelles, including micro-lipid droplets (mLDs), mitochondria, and the endoplasmic reticulum. Exoge? nously added free fatty acids are rapidly adsorbed by mLDs and concurrently get esterified to TG. This process is greatly accelerated by insulin. mLDs transfer their content to the cLD, serving as intermediates that mediate packaging of newly synthesized TG in the large inte? rior of a unilocular adipocyte. This study reveals novel cell biological features that may con? tribute to the mechanism of adipocyte hypertrophy.
Journal Title: Molecular Biology of the Cell
Volume: 25
Issue: 25
ISSN: 1059-1524
Publisher: The American Society for Cell Biology  
Date Published: 2014-12-15
Start Page: 4096
End Page: 4105
Language: English
DOI: 10.1091/mbc.E14-06-1085
PROVIDER: scopus
PMCID: PMC4263452
PUBMED: 25298400
DOI/URL:
Notes: Export Date: 2 January 2015 -- Source: Scopus
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  1. Romel Somwar
    111 Somwar