A versatile scaffold contributes to damage survival via sumoylation and nuclease interactions Journal Article
| Authors: | Sarangi, P.; Altmannova, V.; Holland, C.; Bartosova, Z.; Hao, F.; Anrather, D.; Ammerer, G.; Lee, S. E.; Krejci, L.; Zhao, X. |
| Article Title: | A versatile scaffold contributes to damage survival via sumoylation and nuclease interactions |
| Abstract: | DNA repair scaffolds mediate specific DNA and protein interactions in order to assist repair enzymes inrecognizing and removing damaged sequences. Many scaffold proteins are dedicated to repairing aparticular type of lesion. Here, we show that the budding yeast Saw1 scaffold is more versatile. It helps cells cope with base lesions and protein-DNA adducts through its known function of recruiting the Rad1-Rad10 nuclease to DNA. In addition, it promotes UV survival via a mechanism mediated by its sumoylation. Saw1 sumoylation favors its interaction with another nuclease Slx1-Slx4, and this SUMO-mediated role is genetically separable from two main UV lesion repair processes. These effects ofSaw1 and its sumoylation suggest that Saw1 is a multifunctional scaffold that can facilitate diverse types of DNA repair through its modification and nuclease interactions. |
| Keywords: | signal transduction; controlled study; unclassified drug; nonhuman; protein function; ultraviolet radiation; protein analysis; dna damage; cell survival; dna repair; protein protein interaction; nuclease; double stranded dna break; yeast; sumoylation; fungal protein; protein modification; protein dna interaction; nucleic acid binding protein; protein rad1; article; molecular scaffold; dna repair scaffold; protein rad10; protein saw1; protein slx1; protein slx4 |
| Journal Title: | Cell Reports |
| Volume: | 9 |
| Issue: | 1 |
| ISSN: | 2211-1247 |
| Publisher: | Cell Press |
| Date Published: | 2014-10-09 |
| Start Page: | 143 |
| End Page: | 152 |
| Language: | English |
| DOI: | 10.1016/j.celrep.2014.08.054 |
| PROVIDER: | scopus |
| PUBMED: | 25263559 |
| PMCID: | PMC4280569 |
| DOI/URL: | |
| Notes: | Export Date: 1 December 2014 -- Source: Scopus |
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