Temporospatial coordination of meiotic DNA replication and recombination via DDK recruitment to replisomes Journal Article


Authors: Murakami, H.; Keeney, S.
Article Title: Temporospatial coordination of meiotic DNA replication and recombination via DDK recruitment to replisomes
Abstract: It has been long appreciated that, during meiosis, DNA replication is coordinated with the subsequent formation of the double-strand breaks (DSBs) that initiate recombination, but a mechanistic understanding of this process was elusive. We now show that, in yeast, the replisome-associated components Tof1 and Csm3 physically associate with the Dbf4-dependent Cdc7 kinase (DDK) and recruit it to the replisome, where it phosphorylates the DSB-promoting factor Mer2 in the wake of the replication fork, synchronizing replication with an early prerequisite for DSB formation. Recruiting regulatory kinases to replisomes may be a general mechanism to ensure spatial and temporal coordination of replication with other chromosomal processes.
Keywords: controlled study; protein phosphorylation; unclassified drug; nonhuman; dna replication; chromosome; dna recombination; meiosis; cell cycle s phase; sister chromatid; protein degradation; protein; chromatid; chromatin; double stranded dna break; phosphotransferase; genomic dna; chromosome segregation; pulsed field gel electrophoresis; southern blotting; agarose; replisome; dbf4 dependent cdc7 kinase; dna replication timing; article; protein mer 2; s phase cell cycle checkpoint
Journal Title: Cell
Volume: 158
Issue: 4
ISSN: 0092-8674
Publisher: Cell Press  
Date Published: 2014-08-14
Start Page: 861
End Page: 873
Language: English
DOI: 10.1016/j.cell.2014.06.028
PROVIDER: scopus
PMCID: PMC4141489
PUBMED: 25126790
DOI/URL:
Notes: Export Date: 1 December 2014 -- Source: Scopus
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  1. Scott N Keeney
    107 Keeney