Temporospatial coordination of meiotic DNA replication and recombination via DDK recruitment to replisomes Journal Article

   

Authors:	Murakami, H.; Keeney, S.
Article Title:	Temporospatial coordination of meiotic DNA replication and recombination via DDK recruitment to replisomes
Abstract:	It has been long appreciated that, during meiosis, DNA replication is coordinated with the subsequent formation of the double-strand breaks (DSBs) that initiate recombination, but a mechanistic understanding of this process was elusive. We now show that, in yeast, the replisome-associated components Tof1 and Csm3 physically associate with the Dbf4-dependent Cdc7 kinase (DDK) and recruit it to the replisome, where it phosphorylates the DSB-promoting factor Mer2 in the wake of the replication fork, synchronizing replication with an early prerequisite for DSB formation. Recruiting regulatory kinases to replisomes may be a general mechanism to ensure spatial and temporal coordination of replication with other chromosomal processes.
Keywords:	controlled study; protein phosphorylation; unclassified drug; nonhuman; dna replication; chromosome; dna recombination; meiosis; cell cycle s phase; sister chromatid; protein degradation; protein; chromatid; chromatin; double stranded dna break; phosphotransferase; genomic dna; chromosome segregation; pulsed field gel electrophoresis; southern blotting; agarose; replisome; dbf4 dependent cdc7 kinase; dna replication timing; article; protein mer 2; s phase cell cycle checkpoint
Journal Title:	Cell
Volume:	158
Issue:	4
ISSN:	0092-8674
Publisher:	Cell Press
Date Published:	2014-08-14
Start Page:	861
End Page:	873
Language:	English
DOI:	10.1016/j.cell.2014.06.028
PROVIDER:	scopus
PMCID:	PMC4141489
PUBMED:	25126790
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-84908396303&partnerID=40&md5=140f94c67723e429e1abe29f3a55bdfa
Notes:	Export Date: 1 December 2014 -- Source: Scopus

https://synapse.mskcc.org/synapse/works/has_related_data_chk/65008