AUF1 and Hu proteins in the developing rat brain: Implication in the proliferation and differentiation of neural progenitors Journal Article
| Authors: | Hambardzumyan, D.; Sergent-Tanguy, S.; Thinard, R.; Bonnamain, V.; Masip, M.; Fabre, A.; Boudin, H.; Neveu, I.; Naveilhan, P. |
| Article Title: | AUF1 and Hu proteins in the developing rat brain: Implication in the proliferation and differentiation of neural progenitors |
| Abstract: | Posttranscriptional events such as RNA stabilization are important for cell differentiation, but little is known about the impact of AU-rich binding proteins (AUBPs) on the fate of neural cells. Expression of destabilizing AUBPs such as AUF1 and neuronal-specific stabilizing proteins such as HuB, HuC and HuD was therefore analyzed in the developing central nervous system. Realtime RT-PCR indicated a specific developmental pattern in the postnatal cerebellum, with a progressive down-regulation of AUF1 from P1, whereas HuB was strongly up-regulated at about P7. These changes were accompanied by a progressive increase in AUF1p45 and the disappearance of one HuB isoform from P15, suggesting particular roles for these AUBPs in the developing cerebellum. AUF1 was detected in the three main cerebellar layers, whereas Hu proteins were found only in postmitotic neurons. A role for Hu proteins in the early stages of neuronal differentiation is further supported by arrest of cell proliferation following induction of HuB or HuD expression in a neural stem cell line. The decrease in nestin expression suggest that HuD, but not HuB, favors the transition of neural progenitors into early neuroblasts, but other factors are most probably required for their full differentiation into neurons, insofar as GAP-43 was not detected in HuDtransfected cells. These data suggest critical roles for HuB at the very earliest stages of neuronal differentiation, such as cell cycle exit, and HuD might also be involved in the transition of neural progenitors into early neuroblasts. Taken together, the present results strengthen the importance of AUBPs in brain ontogenesis. © 2008 Wiley-Liss, Inc. |
| Keywords: | controlled study; protein expression; unclassified drug; nonhuman; protein function; cell proliferation; mitosis; animal cell; animal; cytology; metabolism; animals; animal tissue; cerebellum; reverse transcription polymerase chain reaction; cell line; nerve tissue proteins; neural stem cell; neurons; physiology; rna binding protein; gene expression regulation; growth, development and aging; genetic transfection; messenger rna; reverse transcriptase polymerase chain reaction; rna, messenger; brain development; rat; protein induction; cell cycle arrest; development; rats; nerve protein; multipotent stem cell; nerve cell differentiation; nestin; nervous system development; neurogenesis; nerve cell; neural progenitor cells; posttranscriptional regulation; brain protein; heterogeneous nuclear ribonucleoprotein d; hu protein; neuromodulin; protein hub; protein hud; elavl2 protein, rat; elavl3 protein, rat; hnrnp d0; hu antigen; hud protein, rat; intermediate filament protein; isoprotein; developmental stage; nerve regeneration; neuroblast; gap-43 protein; heterogeneous-nuclear ribonucleoprotein d; hu paraneoplastic encephalomyelitis antigens; intermediate filament proteins; multipotent stem cells; protein isoforms |
| Journal Title: | Journal of Neuroscience Research |
| Volume: | 87 |
| Issue: | 6 |
| ISSN: | 0360-4012 |
| Publisher: | Wiley Liss |
| Date Published: | 2009-05-01 |
| Start Page: | 1296 |
| End Page: | 1309 |
| Language: | English |
| DOI: | 10.1002/jnr.21957 |
| PUBMED: | 19115409 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 4" - "Export Date: 30 November 2010" - "CODEN: JNRED" - "Source: Scopus" |
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