Chemical and genetic assessment of variability in commercial Radix Astragali (Astragalus spp.) by ion trap LC-MS and nuclear ribosomal DNA barcoding sequence analyses Journal Article

   

Authors:	Xiao, W. L.; Motley, T. J.; Unachukwu, U. J.; Lau, C. B. S.; Jiang, B.; Hong, F.; Leung, P. C.; Wang, Q. F.; Livingston, P. O.; Cassileth, B. R.; Kennelly, E. J.
Article Title:	Chemical and genetic assessment of variability in commercial Radix Astragali (Astragalus spp.) by ion trap LC-MS and nuclear ribosomal DNA barcoding sequence analyses
Abstract:	Radix Astragali (Huangqi) has been demonstrated to have a wide range of immunopotentiating effects and has been used as an adjuvant medicine during cancer therapy. Identity issues in the collection of Radix Astragali exist because many sympatric species of Astragalus occur in the northern regions of China. In order to assess the quality, purity, and uniformity of commercial Radix Astragali, 44 samples were purchased from herbal stores in Hong Kong and New York City. The main constituents, including four isoflavonoids and three saponins, were quantitatively determined by liquid chromatography mass spectrometry (LC-MS). There was significant sample-to-sample variability in the amounts of the saponins and isoflavonoids measured. Furthermore, DNA barcoding utilizing the variable nuclear ITS spacer regions of the 44 purchased Radix Astragali samples were sequenced, aligned and compared. Eight polymorphic point mutations were identified which separated the Radix Astragali samples into three groups. These results indicate that the chemical and genetic variability that exists among Radix Astragali medicinal products is still a consistency and quality issue for this herbal. Two-way ANOVA analysis showed significant effects on the contents of the seven tested compounds when both phylogenetic and geographic (i.e., point of purchase) factors were considered. Therefore, chemical profiles determined by LC-MS and DNA profiles in ITS spacer domains could serve as barcode markers for quality control of Radix Astragali. © 2011 American Chemical Society.
Keywords:	sequence analysis; mass spectrometry; quality control; genetic variability; cancer therapy; dna; nucleic acids; new york city; astragalus; liquid chromatography; metabolites; dna barcoding; lc-ms; radix astragali; chemical profiles; dna profiles; hong-kong; ion traps; isoflavonoids; liquid chromatography-mass spectrometry; medicinal products; northern regions; point mutations; ribosomal dna; sympatric species; two-way anova; forestry; sales
Journal Title:	Journal of Agricultural and Food Chemistry
Volume:	59
Issue:	5
ISSN:	0021-8561
Publisher:	American Chemical Society
Date Published:	2011-03-09
Start Page:	1548
End Page:	1556
Language:	English
DOI:	10.1021/jf1028174
PROVIDER:	scopus
PMCID:	PMC3058609
PUBMED:	21322556
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-79952159576&partnerID=40&md5=3637255b9a8ced74935252d5e79189e1
Notes:	--- - "Export Date: 23 June 2011" - "CODEN: JAFCA" - "Source: Scopus"

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