Affinity of monoclonal antibodies for Globo-series glycans Journal Article
| Authors: | Eller, C. H.; Yang, G.; Ouerfelli, O.; Raines, R. T. |
| Article Title: | Affinity of monoclonal antibodies for Globo-series glycans |
| Abstract: | Globo-series glycans are human cell-surface carbohydrates that include stem-cell marker SSEA-4 and cancer-cell antigen Globo H. These two hexasaccharides differ only in their terminal saccharide: N-acetylneuraminic acid in SSEA-4 and l-fucose in Globo H. Herein, we evaluated the affinity of the monoclonal antibodies α-SSEA-4 and α-GH for the glycans SSEA-4 and Globo H. Using fluorescence polarization, we find that the two monoclonal antibodies have affinity for their cognate glycan in the low nanomolar range, and have negligible affinity for the non-cognate glycan. Using surface plasmon resonance, we find that each cognate affinity is ∼20-fold greater if the glycan is immobilized on a surface rather than free in solution. We conclude that the terminal saccharide plays a dominant role in the ability of monoclonal antibodies to recognize these Globo-series glycans and that the extraordinary specificity of these antibodies supports their use for identifying and sorting stem-cells (α-SSEA-4) and as an agent in cancer immunotherapy (α-GH). © 2014 Published by Elsevier Ltd. |
| Keywords: | unclassified drug; human cell; binding affinity; antigen expression; cancer immunotherapy; clinical assessment; protein binding; fluorescence polarization; tumor antigen; stem cell; monoclonal antibodies; monoclonal antibody; antigens; stem cells; antibody specificity; cross reaction; structure analysis; polysaccharides; surface plasmon resonance; diseases; synthesis; hexasaccharides; glycans; glycan derivative; carbohydrate; antibody affinity; cell surface; surface property; biomedicine; binding assay; n acetylneuraminic acid; stage specific embryo antigen 4; globo h; human; priority journal; article; cancer-cell antigen; globo-series glycan; ssea-4; stem-cell marker; n-acetylneuraminic acid; nanomolar range; growth hormone alpha; stage specific embryo antigen 4 alpha; fucose; monoclonal antibody globo h alpha; monoclonal antibody stage specific embryo antigen 4 alpha |
| Journal Title: | Carbohydrate Research |
| Volume: | 397 |
| ISSN: | 0008-6215 |
| Publisher: | Elsevier Science, Inc. |
| Date Published: | 2014-10-09 |
| Start Page: | 1 |
| End Page: | 6 |
| Language: | English |
| DOI: | 10.1016/j.carres.2014.07.003 |
| PROVIDER: | scopus |
| PUBMED: | 25163606 |
| PMCID: | PMC4237592 |
| DOI/URL: | |
| Notes: | Export Date: 1 October 2014 -- Source: Scopus |
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