Melphalan-mobilized blood stem cell components contain minimal clonotypic myeloma cell contamination Journal Article
| Authors: | Zhou, P.; Zhang, Y.; Martinez, C.; Kalakonda, N.; Nimer, S. D.; Comenzo, R. L. |
| Article Title: | Melphalan-mobilized blood stem cell components contain minimal clonotypic myeloma cell contamination |
| Abstract: | Optimal methods of stem cell mobilization in multiple myeloma are undefined, and contaminating clonotypic cells could contribute to disease recurrence. A phase 2 trial of intravenous melphalan (60 mg/m2) and granulocyte colony-stimulating factor (G-CSF) (10 μg/kg/d) for mobilization was performed. To enhance reliability, contamination was assessed with 2 sensitive methods, immunoglobulin light and heavy chain variable region patient-specific limiting-dilution polymerase chain reaction (PCR). We evaluated 29 stem cell components (SCCs) from 15 patients; for 9 SCCs, only VL PCR was used because of light chain disease or technical problems with VH primers. For 20 SCCs, VL and VH PCR results were highly correlated (r2 = 0.93, P < .01), with 35% (7 of 20) having identical estimates. VH PCR gave significantly higher estimates for 8 - and VL PCR for 5 - SCCs, supporting the utility of using 2 methods. Estimated clonotypic contamination per SCC was 0.0009% (range, 0%-0.1%) or 0.5 × 104 clonotypic cells per kilogram (range, 0-41.2 x 104/kg), and contamination correlated with CD34+ cells collected (r2 = 0.42, P < .01). Melphalan-mobilized SCCs contain minimal clonotypic contamination. © 2003 by The American Society of Hematology. |
| Keywords: | adult; clinical article; controlled study; aged; middle aged; human cell; clinical trial; sensitivity and specificity; polymerase chain reaction; cell function; controlled clinical trial; multiple myeloma; phase 2 clinical trial; melphalan; immunoglobulin g; stem cell mobilization; recurrent disease; intermethod comparison; reliability; immunoglobulin light chain; granulocyte colony stimulating factor; granulocyte colony-stimulating factor; hematopoietic stem cell mobilization; clone cells; immunoglobulin a; leukapheresis; somatic hypermutation, immunoglobulin; myeloma cell; neoplasm circulating cells; myeloma proteins; gene rearrangement, b-lymphocyte, heavy chain; humans; human; male; female; priority journal; article; gene rearrangement, b-lymphocyte, light chain |
| Journal Title: | Blood |
| Volume: | 102 |
| Issue: | 2 |
| ISSN: | 0006-4971 |
| Publisher: | American Society of Hematology |
| Date Published: | 2003-07-15 |
| Start Page: | 477 |
| End Page: | 479 |
| Language: | English |
| DOI: | 10.1182/blood-2002-12-3674 |
| PUBMED: | 12649134 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 12 September 2014 -- Source: Scopus |
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