Antitumor effect of intratumoral administration of dendritic cell combination with vincristine chemotherapy in a murine fibrosarcoma model Journal Article
| Authors: | Shin, J. Y.; Lee, S. K.; Kang, C. D.; Chung, J. S.; Lee, E. Y.; Seo, S. Y.; Lee, S. Y.; Baek, S. Y.; Kim, B. S.; Kim, J. B.; Yoon, S. |
| Article Title: | Antitumor effect of intratumoral administration of dendritic cell combination with vincristine chemotherapy in a murine fibrosarcoma model |
| Abstract: | A new antitumor therapeutic strategy utilizing the combined effect of chemotherapy and DC (dendritic cell)-based immunotherapy was designed, and the effect of intratumoral injections of unpulsed, immature DCs was evaluated after in vivo pretreatment of vincristine on tumor growth in a murine fibrosarcoma tumor model. Vincristine exerted a much more potent apoptosis/necrosis-inducing effect on MCA-102 tumor cells than on DCs both in vitro and in vivo. Moreover, CD 11c, CD40, CD80 and CD86 molecules on DCs were not downregulated after treatment with vincristine either in vitro or in vivo. The growth of tumor significantly regressed in the group which received the combined vincristine chemotherapy with intratumoral administration of DCs in contrast to the untreated group, the group treated with DCs alone, and the group treated with vincristine alone. In particular, an upregulated expression of CD40, CD80 and CD86 molecules on DCs was found in the combination treatment group. Furthermore, the number of CD4+ and CD8+ T cells and the staining intensity of their CD4 and CDS surface molecules also increased after the combination treatment. Therefore, our results indicate the feasibility of this combination therapy with vincristine chemotherapy and DC-based immunotherapy as an efficient antitumor strategy for the treatment of fibrosarcoma. |
| Keywords: | immunohistochemistry; cancer chemotherapy; controlled study; cisplatin; doxorubicin; fluorouracil; multimodality cancer therapy; nonhuman; combined modality therapy; chemotherapy; flow cytometry; cd8 antigen; antigen expression; t lymphocyte; cd8-positive t-lymphocytes; animal cell; mouse; phenotype; animals; mice; animal tissue; cancer immunotherapy; apoptosis; dendritic cell; etoposide; animal experiment; animal model; antineoplastic agents, phytogenic; cyclophosphamide; vincristine; in vivo study; antineoplastic activity; drug potency; in vitro study; drug effect; tumor cell culture; tumor cells, cultured; necrosis; mice, inbred c57bl; ifosfamide; vinblastine; animalia; cytokines; dendritic cells; feasibility study; statistical analysis; fibrosarcoma; immunotherapy; cancer cell; cd4-positive t-lymphocytes; staining; murinae; bleomycin; cd4 antigen; tumor growth; thiazoles; drug utilization; b7 antigen; cd86 antigen; cell transplantation; tetrazolium salts; tumor immunology; in situ nick-end labeling; cd40 antigen; glycoprotein p 15095; female; article; apoptotic/necrotic tumor cells; dendritic cell-based tumor immunotherapy |
| Journal Title: | Histology and Histopathology |
| Volume: | 18 |
| Issue: | 2 |
| ISSN: | 0213-3911 |
| Publisher: | Thomson Scientific |
| Date Published: | 2003-04-01 |
| Start Page: | 435 |
| End Page: | 447 |
| Language: | English |
| PUBMED: | 12647794 |
| PROVIDER: | scopus |
| DOI: | 10.14670/HH-18.435 |
| DOI/URL: | |
| Notes: | Export Date: 12 September 2014 -- Source: Scopus |
