Studies with cytotoxic agents suggest that apoptosis is not a major determinant of clonogenic death in neuroblastoma cells Journal Article
| Authors: | Russell, J.; Ling, C. C. |
| Article Title: | Studies with cytotoxic agents suggest that apoptosis is not a major determinant of clonogenic death in neuroblastoma cells |
| Abstract: | We have previously reported the selection of a radioresistant human neuroblastoma cell line, Clone F, from IMR32 cells. We have shown that clonogenic radioresistance in these cells is accompanied by a reduced level of radiation-induced apoptosis [Cancer Res 55 (1995) 4915]. Here, we measured the response of these lines to several cytotoxic agents, in terms of clonogenicity and apoptosis. In the clonogenic assay, the radioresistant line was also resistant to cisplatin, melphalan and doxorubicin, but not to perillyl alcohol. However, all these agents produced less apoptosis in the Clone F cells, except cisplatin, which failed to induce any apoptosis in either cell line. Reduced apoptosis cannot be the cause of the Clone F cells' resistance to cisplatin. By extension, the Clone F cells' resistance to radiation and other agents cannot be due to diminished apoptosis either. Based on these results, apoptosis may not be a useful surrogate for clonogenic outcome. © 2003 Elsevier Ltd. All rights reserved. |
| Keywords: | controlled study; human cell; cisplatin; cytotoxic agent; doxorubicin; antineoplastic agents; cell survival; apoptosis; radiation; melphalan; cancer cell culture; drug effect; dose-response relationship, drug; drug resistance, neoplasm; cell line, tumor; dose-response relationship, radiation; molecular cloning; blotting, western; neuroblastoma; neuroblastoma cell; tamoxifen; clonogenic assay; clonogenesis; radiation tolerance; tumor stem cell assay; genes, bcl-2; humans; human; priority journal; article; perillyl alcohol; monoterpenes |
| Journal Title: | European Journal of Cancer |
| Volume: | 39 |
| Issue: | 15 |
| ISSN: | 0959-8049 |
| Publisher: | Elsevier Inc. |
| Date Published: | 2003-10-01 |
| Start Page: | 2234 |
| End Page: | 2238 |
| Language: | English |
| DOI: | 10.1016/s0959-8049(03)00488-x |
| PUBMED: | 14522384 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 12 September 2014 -- Source: Scopus |
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