Immunohistochemical staining of cytologic smears with MIB-1 helps distinguish low-grade from high-grade neuroendocrine neoplasms Journal Article

  

Authors:	Lin, O.; Olgac, S.; Green, I.; Zakowski, M. F.; Klimstra, D. S.
Article Title:	Immunohistochemical staining of cytologic smears with MIB-1 helps distinguish low-grade from high-grade neuroendocrine neoplasms
Abstract:	Neuroendocrine neoplasms (NENs) of the lung and gastrointestinal tract constitute a pathologic and biologic spectrum of tumors. Accurate cytologic diagnosis of a neuroendocrine neoplasm is important since definitive treatment frequently is based on low- and high-grade categories without histologic sampling. In many instances, however, low- and high-grade NENs share cytologic features, hindering a precise classification. Since the histologic diagnostic criteria for separation of low- from high-grade categories can be based on the proliferation rate, we proposed to evaluate the usefulness of the immunocytochemical stain for the proliferation marker MIB-1 in the grading of NENs. Cytologic preparations of 63 NENs were retrieved from the files of Memorial Sloan-Kettering Cancer Center, New York, NY. One representative alcohol-fixed slide from each case was destained and restained immunocytochemically for MIB-1. When MIB-1 immunoreactivity was considered, all low-grade NENs showed immunoreactivity in fewer than 25% of the neoplastic cells, and all high-grade NENs demonstrated immunoreactivity in more than 50% of neoplastic cells. Our study demonstrates that MIB-1 dramatically stratifies NENs as low-grade or high-grade. Therefore, the proliferation index also correlates with grade of NEN in cytology specimens.
Keywords:	immunohistochemistry; adult; controlled study; human tissue; aged; aged, 80 and over; middle aged; major clinical study; liver neoplasms; methodology; cancer diagnosis; cancer grading; diagnostic accuracy; neoplasms; ki 67 antigen; cell proliferation; ki-67 antigen; reproducibility; reproducibility of results; cytology; metabolism; metastasis; classification; diagnosis, differential; lung neoplasms; differential diagnosis; tumor markers, biological; cell differentiation; pathology; immunoreactivity; tumor marker; neuroendocrine tumor; lung tumor; correlation analysis; evaluation; immunocytochemistry; liver tumor; carcinoma; lung carcinoma; reliability; proliferation; tumor classification; cytodiagnosis; carcinoma, neuroendocrine; neuroendocrine; mib-1; gastrointestinal carcinoma; humans; human; male; female; priority journal; article
Journal Title:	American Journal of Clinical Pathology
Volume:	120
Issue:	2
ISSN:	0002-9173
Publisher:	Oxford University Press
Date Published:	2003-08-01
Start Page:	209
End Page:	216
Language:	English
DOI:	10.1309/tgcd66l31dhyx5hk
PUBMED:	12931551
PROVIDER:	scopus
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-0042885369&partnerID=40&md5=dc31175e13acea767a4af08715b4e0b1
Notes:	Export Date: 12 September 2014 -- Source: Scopus

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