Bcl-2 and bax expression in advanced non-small cell lung cancer: Lack of correlation with chemotherapy response or survival in patients treated with docetaxel plus vinorelbine Journal Article

  

Authors:	Krug, L. M.; Miller, V. A.; Filippa, D. A.; Venkatraman, E.; Ng, K. K.; Kris, M. G.
Article Title:	Bcl-2 and bax expression in advanced non-small cell lung cancer: Lack of correlation with chemotherapy response or survival in patients treated with docetaxel plus vinorelbine
Abstract:	Surgical series of non-small cell lung cancer (NSCLC) pathologic samples have shown that the expression of the proteins bcl-2 and bax, which regulate cell death, may have prognostic implications. Laboratory data also suggests that these proteins may impact chemotherapy response. In order to determine the rate of bcl-2 and bax expression in advanced NSCLC and assess the impact on chemotherapy response and patient survival, we performed immunohistochemistry on biopsy samples from patients enrolled in a phase I/II trial of vinorelbine plus docetaxel. We chose to study the pathology of patients in this specific trial because both docetaxel and vinorelbine phosphorylate bcl-2 and we hypothesized that this mechanism may affect clinical outcome. The goal of this study was to determine the feasibility of this analysis, and to observe any differences in response rate or survival based on bcl-2 or bax staining results. Unstained slides from paraffin blocks were obtained for 31 patients (55%) on the phase I/II trial. The patient characteristics for this subgroup did not differ significantly from the entire cohort of patients on the trials. Bcl-2 staining was positive in 5/31 samples (16%, 95% CI 3-29%) and bax was positive in 19/28 samples (68%, 95% CI 51-85%). Bcl-2 and bax staining did not correlate with response (p=0.65 and 1.00 respectively, Fisher's exact test), or survival (by Kaplan-Meier curves). In conclusion, bcl-2 and bax expression was similar in this population with advanced NSCLC to previously reported results for early stage disease, but did not predict response to vinorelbine plus docetaxel in this series. © 2002 Elsevier Science Ireland Ltd. All rights reserved.
Keywords:	immunohistochemistry; cancer survival; controlled study; human tissue; protein expression; survival rate; proto-oncogene proteins; clinical trial; advanced cancer; paclitaxel; chemotherapy; neoplasm staging; protein bcl 2; controlled clinical trial; phase 2 clinical trial; cohort studies; lung non small cell cancer; antineoplastic combined chemotherapy protocols; carcinoma, non-small-cell lung; lung neoplasms; tumor markers, biological; biopsy; vinblastine; docetaxel; feasibility study; staining; taxoids; phase 1 clinical trial; kaplan meier method; navelbine; fisher exact test; proto-oncogene proteins c-bcl-2; non-small cell lung cancer; bax; bcl-2; protein bax; bcl-2-associated x protein; vinorelbine; humans; prognosis; human; male; female; priority journal; article
Journal Title:	Lung Cancer
Volume:	39
Issue:	2
ISSN:	0169-5002
Publisher:	Elsevier Ireland Ltd.
Date Published:	2003-02-01
Start Page:	139
End Page:	143
Language:	English
DOI:	10.1016/s0169-5002(02)00443-9
PUBMED:	12581565
PROVIDER:	scopus
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-0037312094&partnerID=40&md5=a5b3790c900fc980164d8e83f1187d88
Notes:	Export Date: 12 September 2014 -- Source: Scopus

https://synapse.mskcc.org/synapse/works/has_related_data_chk/59772