The BRCA2-interacting protein DSS1 is vital for DNA repair, recombination, and genome stability in Ustilago maydis Journal Article

  

Authors:	Kojic, M.; Yang, H.; Kostrub, C. F.; Pavletich, N. P.; Holloman, W. K.
Article Title:	The BRCA2-interacting protein DSS1 is vital for DNA repair, recombination, and genome stability in Ustilago maydis
Abstract:	DSS1 encodes a small acidic protein shown in recent structural studies to interact with the DNA binding domain of BRCA2. Here we report that an ortholog of DSS1 is present in Ustilago maydis and associates with Brh2, the BRCA2-related protein, thus recapitulating the protein partnership in this genetically amenable fungus. Mutants of U. maydis deleted of DSS1 are extremely radiation sensitive, deficient in recombination, defective in meiosis, and disturbed in genome stability; these phenotypes mirror previous observations of U. maydis mutants deficient in Brh2 or Rad51. These findings conclusively show that Dss1 constitutes a protein with a significant role in the recombinational repair pathway in U. maydis, and imply that it plays a similar key role in the recombination systems of organisms in which recombinational repair is BRCA2 dependent.
Keywords:	controlled study; unclassified drug; mutation; dna-binding proteins; nonhuman; proteins; dna recombination; meiosis; dna repair; protein protein interaction; protein stability; brca2 protein; evolution, molecular; dna; molecular sequence data; sequence homology, amino acid; recombination, genetic; genomic instability; nucleotide sequence; genome; protein deficiency; sequence homology, nucleic acid; fungi; fungal protein; rad51 protein; mutant; rad51 recombinase; protein brh2; ustilago maydis; fungal proteins; orthology; ustilago; article; protein dss1
Journal Title:	Molecular Cell
Volume:	12
Issue:	4
ISSN:	1097-2765
Publisher:	Cell Press
Date Published:	2003-10-01
Start Page:	1043
End Page:	1049
Language:	English
DOI:	10.1016/s1097-2765(03)00367-8
PUBMED:	14580353
PROVIDER:	scopus
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-0242361315&partnerID=40&md5=839d4b31450991838f3896772d1831cc
Notes:	Export Date: 12 September 2014 -- Molecular Sequence Numbers: GENBANK: AAD08804, AY366406, NM_120241, NP_006295; -- Source: Scopus

https://synapse.mskcc.org/synapse/works/has_related_data_chk/59757