Insights into extramedullary tumour cell growth revealed by expression profiling of human plasmacytomas and multiple myeloma Journal Article

Authors: Hedvat, C. V.; Comenzo, R. L.; Teruya-Feldstein, J.; Olshen, A. B.; Ely, S. A.; Osman, K.; Zhang, Y.; Kalakonda, N.; Nimer, S. D.
Article Title: Insights into extramedullary tumour cell growth revealed by expression profiling of human plasmacytomas and multiple myeloma
Abstract: Malignant plasma cells generally grow within the bone marrow microenvironment; however, they can also grow at extramedullary sites. To identify the tumour-specific alterations required for extramedullary growth, we analysed the expression profiles of a series of plasma cell neoplasms including primary multiple myeloma (MM), plasma cell leukaemia (PCL) and extramedullary plasmacytoma (EPC). Hierarchical clustering analysis segregated the EPCs from the remaining samples, and revealed an expression pattern associated with angiogenesis in the EPCs, involving higher expression of the genes TIE2, NOTCH3, CD31 and endoglin. Direct comparison of EPC samples with the MM samples identified 156 genes significantly upregulated and 85 genes significantly downregulated (P < 0.005, t-test) in the EPCs, including several genes involved in angiogenesis and adhesion that were upregulated (including angiopoietin 1. SPARC, Notch3 and fibronectin 1). Immunohistochemical staining demonstrated CD31 and endoglin protein expression in the EPC tumour cells, which are both angiogenesis related and could confer malignant plasma cells with the ability to grow outside the normal bone marrow environment. Defining how malignant plasma cell growth is regulated in the bone marrow versus at extramedullary sites will help to delineate the mechanisms underlying the dependence of tumour cell growth on angiogenesis and cell adhesion.
Keywords: immunohistochemistry; human tissue; protein expression; human cell; cell survival; cell division; multiple myeloma; cluster analysis; gene expression profiling; bone marrow; plasmacytoma; tumor cells, cultured; angiogenesis; neovascularization, pathologic; plasma cell; oligonucleotide array sequence analysis; nucleotide sequence; cancer cell; down regulation; upregulation; tumor growth; cell adhesion; unindexed sequence; myeloma; humans; human; priority journal; article; gene arrays; myeloma cell lines; plasma cell leukemia
Journal Title: British Journal of Haematology
Volume: 122
Issue: 5
ISSN: 0007-1048
Publisher: John Wiley & Sons  
Date Published: 2003-09-01
Start Page: 728
End Page: 744
Language: English
DOI: 10.1046/j.1365-2141.2003.04481.x
PUBMED: 12930383
PROVIDER: scopus
Notes: Export Date: 12 September 2014 -- Molecular Sequence Numbers: GENBANK: AB002364, AB005060, AB008109, AB011792, AF018081, AF041037, AF093119, AI651806, AJ001015, AL049415, D11151, D87258, J03040, J03278, J03464, L12350, L13210, L19182, L27560, L39833, M26576, M26683, M30269, M31682, M35878, M62403, M69023, M85289, M96789, S78569, U10550, U14394, U21128, U26424, U41766, U58516, U97669, X02761, X05610, X06374, X14420, X45882, X52022, X52947, X53002, X78565, X79981, X86693, X87241, Y14690 -- Source: Scopus
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MSK Authors
  1. Cyrus Hedvat
    123 Hedvat
  2. Julie T Feldstein
    288 Feldstein
  3. Keren Osman
    8 Osman
  4. Yana Zhang
    12 Zhang
  5. Raymond L Comenzo
    112 Comenzo
  6. Adam B Olshen
    107 Olshen
  7. Stephen D Nimer
    345 Nimer