The role of lipopolysaccharide in T-cell responses following DNA vaccination Journal Article
| Authors: | Hawkins, W. G.; Trcka, J.; Segal, N.; Blachere, N. E.; Gold, J. S.; Moroi, Y.; Bowne, W. B.; Lewis, J. J.; Wolchok, J. D.; Houghton, A. N. |
| Article Title: | The role of lipopolysaccharide in T-cell responses following DNA vaccination |
| Abstract: | Bacterial products, including lipopolysaccharide (LPS), are potential impurities in plasmid DNA vaccines. LPS has immunostimulatory properties even at exceedingly low concentrations through activation of Toll-like receptor 4 (TLR4). The potency of T-cell responses after vaccination was tested with DNA containing high LPS or depleted of LPS in TLR4-competent and TLR4-deficient mice. CD8+ T-cell responses were readily induced in TLR4-deficient mice immunized with DNA depleted of LPS. LPS in DNA vaccines is not required for CD8+ T-cell responses. © 2002 Elsevier Science Ltd. All rights reserved. |
| Keywords: | controlled study; nonhuman; protein function; cd8 antigen; t lymphocyte; cd8-positive t-lymphocytes; animal cell; mouse; animals; mice; animal tissue; animal experiment; strain difference; mice, inbred balb c; mice, inbred c57bl; toll like receptor 4; dendritic cells; cellular immunity; immune response; membrane glycoproteins; vaccination; lipopolysaccharide; dna vaccine; immunostimulation; mouse strain; vaccines, dna; receptors, cell surface; toll-like receptors; immunization; mice, inbred c3h; toll-like receptor 4; lipopolysaccharides; t-cell immunity; priority journal; article; dna vaccination; vaccination reaction |
| Journal Title: | Vaccine |
| Volume: | 21 |
| Issue: | 13-14 |
| ISSN: | 0264-410X |
| Publisher: | Elsevier Inc. |
| Date Published: | 2003-03-28 |
| Start Page: | 1548 |
| End Page: | 1553 |
| Language: | English |
| DOI: | 10.1016/s0264-410x(02)00676-x |
| PUBMED: | 12615452 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 12 September 2014 -- Source: Scopus |
