Association of DNA quadruplexes through G:C:G:C tetrads. Solution structure of d(GCGGTGGAT) Journal Article
| Author: | da Silva, M. W. |
| Article Title: | Association of DNA quadruplexes through G:C:G:C tetrads. Solution structure of d(GCGGTGGAT) |
| Abstract: | The structure formed by the DNA sequence d(GCGGTGGAT) in a 100 mM Na + solution has been determined using molecular dynamics calculations constrained by distance and dihedral restraints derived from NMR experiments performed at isotopic natural abundance. The sequence folds into a dimer of dimers. Each symmetry-related half contains two parallel stranded G:G:G:G tetrads flanked by an A:A mismatch and by four-stranded G:C:G:C tetrads. Each of the two juxtaposed G:C:G:C tetrads is composed of alternating antiparallel strands from the two halves of the dimer. For each single strand, a thymine intersperses a double chain reversal connecting the juxtaposed G:G:G:G tetrads. This architecture has potential implications in genetic recombination. It suggests a pathway for oligomerization involving association of quadruplex entities through GpC steps. |
| Keywords: | sequence analysis; protein localization; molecular dynamics; dna; genetic recombination; genetic engineering; guanine; recombination, genetic; nuclear magnetic resonance spectroscopy; base sequence; dna flanking region; dna sequence; dna structure; hydrogen bonding; protons; solutions; thermodynamics; molecular structure; molecular interaction; nucleic acid conformation; protein folding; structure analysis; biochemistry; sodium; sodium chloride; oligomerization; nucleotide repeat; oligonucleotides; calculation; adenine; nuclear magnetic resonance, biomolecular; base mispairing; thymine; dimer; dna strand; cytosine; base composition; priority journal; article |
| Journal Title: | Biochemistry |
| Volume: | 42 |
| Issue: | 49 |
| ISSN: | 0006-2960 |
| Publisher: | American Chemical Society |
| Date Published: | 2003-12-16 |
| Start Page: | 14356 |
| End Page: | 14365 |
| Language: | English |
| DOI: | 10.1021/bi0355185 |
| PUBMED: | 14661946 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 12 September 2014 -- Source: Scopus |
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