Catalytic properties of ADAM19 Journal Article
| Authors: | Chesneau, V.; Becherer, J. D.; Zheng, Y.; Erdjument-Bromage, H.; Tempst, P.; Blobel, C. P. |
| Article Title: | Catalytic properties of ADAM19 |
| Abstract: | ADAMs are membrane-anchored glycoproteins with functions in fertilization, heart development, neurogenesis, and protein ectodomain shedding. Here we report an evaluation of the catalytic activity of recombinantly expressed soluble forms of ADAM19, a protein that is essential for cardiovascular morphogenesis. Proteolytic activity of soluble forms of ADAM19 was first demonstrated by their autocatalytic removal of a purification tag (Myc-His) and their ability to cleave myelin basic protein and the insulin B chain. The metalloprotease activity of ADAM19 is sensitive to the hydroxamic acid-type metalloprotease inhibitor BB94 (batimastat) but not to tissue inhibitors of metalloproteases (TIMPs) 1-3. Moreover, ADAM19 cleaves peptides corresponding to the known cleavage sites of tumor necrosis factor-(TNF-α), TNF-related activation-induced cytokine (TRANCE, also referred to as osteoprotegerin ligand), and kit ligand-1 (KL-1) in vitro. Although ADAM19 is not required for shedding of TNFα and TRANCE in mouse embryonic fibroblasts, its overexpression in COS-7 cells results in strongly increased TRANCE shedding. This suggests a potential role for ADAM19 in shedding TRANCE in cells where both molecules are highly expressed, such as in osteoblasts. Interestingly, our results also indicate that ADAM19 can function as a negative regulator of KL-1 shedding in both COS-7 cells and mouse embryonic fibroblasts, instead of acting directly on KL-1. The identification of potential in vitro substrates offers the basis for further functional studies of ADAM19 in cells and in mice. |
| Keywords: | protein expression; unclassified drug; nonhuman; protein function; proteins; myelin basic protein; animal cell; animals; mice; mice, knockout; gene overexpression; enzyme inhibition; protein degradation; cell line; protein; membrane proteins; enzyme activity; transfection; cell strain cos7; cercopithecus aethiops; cos cells; animalia; amino acid sequence; molecular sequence data; tumor necrosis factor alpha; kinetics; genetic engineering; nucleotide sequence; recombinant proteins; membrane protein; recombinant protein; ligand; fibroblast; fibroblasts; insulin; spodoptera; binding sites; catalysis; animal cell culture; nervous system development; heart development; metalloproteinase; osteoclast differentiation factor; adam proteins; catalyst activity; tissue inhibitor of metalloproteinase 1; tissue inhibitor of metalloproteinase 2; tissue inhibitor of metalloproteinase 3; regulator protein; chemical activation; protein ectodomain shedding; metalloproteases; biological membranes; metalloproteinase inhibitor; muscle proteins; protein adam19; fertilization; disintegrins; metalloendopeptidases; priority journal; article; batimastat; insulin b chain; kit ligand 1; oncogene c myc histidine tagged protein; autocatalysis |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 278 |
| Issue: | 25 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 2003-06-20 |
| Start Page: | 22331 |
| End Page: | 22340 |
| Language: | English |
| DOI: | 10.1074/jbc.M302781200 |
| PUBMED: | 12682046 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 12 September 2014 -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
MSK Authors
Related MSK Work