Expression of protein C inhibitor (PCI) in benign and malignant prostatic tissues Journal Article
| Authors: | Cao, Y.; Becker, C.; Lundwall, Å; Christensson, A.; Gadaleanu, V.; Lilja, H.; Bjartell, A. |
| Article Title: | Expression of protein C inhibitor (PCI) in benign and malignant prostatic tissues |
| Abstract: | BACKGROUND. Protein C inhibitor (PCI) occurs at high concentration in seminal plasma, and inhibits human glandular kallikrein-2 and, less readily, prostate-specific antigen. Previous studies have localized PCI in the male genital tract. Here we have performed a detailed investigation of PCI expression in the prostatic tissues, metastases, and cell lines. METHODS. Immunohistochemistry, in situ hybridization, and Western blotting were used to study prostatic tissues, metastases, and PC-3, DU-145, and LNCaP cells. RESULTS. PCI was immunolocalized in tissue microarray spots with BPH epithelium (detection rate 100%), PIN lesions (100%), tumors (96%), metastases (88%), and in all cell lines. ISH and WB supported the findings. CONCLUSIONS. PCI is widely expressed in benign prostatic epithelium, and may act as a local regulator of enzymatic activity in seminal fluid, of importance for normal sperm function. Lack of PCI expression in a subpopulation of high-grade tumor cells in combination with maintained protease expression may facilitate invasive growth patterns. © 2003 Wiley-Liss, Inc. |
| Keywords: | immunohistochemistry; controlled study; human tissue; protein expression; human cell; major clinical study; cancer growth; nonhuman; cancer grading; sensitivity and specificity; protein localization; prostate specific antigen; mouse; metastasis; cancer cell culture; enzyme activity; cell line, tumor; prostate cancer; cancer invasion; prostate-specific antigen; prostatic neoplasms; in situ hybridization; blotting, western; prostate; regulatory mechanism; cancer cell; western blotting; cell subpopulation; carcinoma; kallikrein; prostate epithelium; prostate hypertrophy; dna microarray; prostatic hyperplasia; proteinase; tissue microarray; rna, neoplasm; androgens; plasma protein; neoplasms, hormone-dependent; seminal plasma; sperm; cell lines; human glandular kallikrein-2; plasminogen activator inhibitor 3; immunolocalization; humans; human; male; priority journal; article; serpin; protein c inhibitor |
| Journal Title: | Prostate |
| Volume: | 57 |
| Issue: | 3 |
| ISSN: | 0270-4137 |
| Publisher: | John Wiley & Sons |
| Date Published: | 2003-11-01 |
| Start Page: | 196 |
| End Page: | 204 |
| Language: | English |
| DOI: | 10.1002/pros.10296 |
| PUBMED: | 14518028 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 12 September 2014 -- Source: Scopus |
