The alternative Ctf18-Dcc1-Ctf8-replication factor C complex required for sister chromatid cohesion loads proliferating cell nuclear antigen onto DNA Journal Article

   


Authors: Bermudez, V. P.; Maniwa, Y.; Tappin, I.; Ozato, K.; Yokomori, K.; Hurwitz, J.
Article Title: The alternative Ctf18-Dcc1-Ctf8-replication factor C complex required for sister chromatid cohesion loads proliferating cell nuclear antigen onto DNA
Abstract: The linkage of sister chromatids after DNA replication ensures the faithful inheritance of chromosomes by daughter cells. In budding yeast, the establishment of sister chromatid cohesion requires Ctf8, Dcc1, and Ctf18, a homologue of the p140 subunit of the replication factor C (RFC). In this report we demonstrate that in 293T cells, Flag-tagged Ctf18 forms a seven-subunit cohesion-RFC complex comprised of Ctf18, Dcc1, Ctf8, RFCp40, RFCp38, RFCp37, and RFCp36 (Ctf18-RFC). We demonstrate that a stoichiometric hetero-heptameric Ctf18-RFC complex can be assembled by coexpressing the seven proteins in baculovirus-infected insect cells. In addition, the two other stable subcomplexes were formed, which include a pentameric complex comprised of Ctf18, RFCp40, RFCp38, RFCp37, and RFCp36 and a dimeric Dcc1-Ctf8. Both the five- and seven-subunit Ctf18-RFC complexes bind to single-stranded and primed DNAs and possess weak ATPase activity that is stimulated by the addition of primed DNA and proliferating cell nuclear antigen (PCNA). These complexes catalyzed the ATP-dependent loading of PCNA onto primed and gapped DNA but not onto double-stranded nicked or single-stranded circular DNAs. Consistent with these observations, both Ctf18-RFC complexes substituted for the replicative RFC in the PCNA-dependent DNA polymerase δ-catalyzed DNA replication reaction. These results support a model in which sister chromatid cohesion is linked to DNA replication.
Keywords: unclassified drug; dna-binding proteins; nonhuman; dna polymerase; dna replication; animal cell; complex formation; sister chromatid; cell line; protein; animalia; chromatid; nuclear proteins; dna; nucleotide sequence; carrier proteins; yeast; saccharomycetales; catalysis; replication factor c; adenosine triphosphate; cycline; protein subunit; single stranded dna; replication protein c; sequence homology; adenosine triphosphatases; genetic linkage; chromatids; proliferating cell nuclear antigen; dimer; dna polymerase iii; unidentified baculovirus; circular dna; insecta; humans; priority journal; article; ctf18 protein; ctf8 protein; dcc1 protein
Journal Title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 100
Issue: 18
ISSN: 0027-8424
Publisher: National Academy of Sciences  
Date Published: 2003-09-02
Start Page: 10237
End Page: 10242
Language: English
DOI: 10.1073/pnas.1434308100
PUBMED: 12930902
PROVIDER: scopus
PMCID: PMC193545
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-0042337435&partnerID=40&md5=08aee342d9f031a700ecdd9deff0201d
Notes: Export Date: 12 September 2014 -- Molecular Sequence Numbers: GENBANK: BC001316, XM_027413; -- Source: Scopus
Altmetric
What is Altmetric?
Citation Impact
What is Dimensions Citation Badge?
BMJ Impact Analytics
MSK Authors
  1. Yoshimasa Maniwa
    2 Maniwa
  2. Vladimir D Bermudez
    32 Bermudez
  3. Jerard Hurwitz
    206 Hurwitz
  4. Inger Tappin
    15 Tappin
Related MSK Work