| Abstract: |
Papillary thyroid carcinomas (PTC) are the most common thyroid cancers in children. Most are successfully treated with surgery and radioactive iodine, but some persist. PTC express high levels of vascular endothelial growth factor (VEGF) and VEGF receptor (Flt-1). PTC with the most intense expression of VEGF have the greatest recurrence risk. We hypothesized that blockade of VEGF would inhibit PTC growth. To test this, we used systemic VEGF monoclonal antibody (VEGF-MAb) to treat PTC xenografts in nude mice. Treated animals (n = 9) received 200 μg VEGF-MAb by daily ip injection for 10 wk, while control animals (n = 9) received vehicle alone. Tumor size was significantly reduced in the treatment group (0.28 ± 0.06 vs 1.05 ± 0.25 g, p = 0.008). VEGF immunostaining was more intense (2.57 ± 0.30 vs 1.75 ± 0.25, p = 0.06) and the number of p53 positive cells was increased (1.66 ± 0.24 vs 0.83 ± 0.3 1, p = 0.048) in treated tumors. Animal weight was similar in both groups (29.1 ± 1.1 vs 27.4 ± 1.1 g, p = 0.30). In conclusion, systemic VEGF-MAb significantly reduced the growth of PTC, suggesting that VEGF-MAb might be useful for treatment of resistant PTC. |
| Keywords: |
immunohistochemistry; vasculotropin; controlled study; protein expression; vascular endothelial growth factor a; cancer growth; nonhuman; recurrence risk; animal cell; mouse; animals; mice; animal tissue; vasculotropin receptor; cancer immunotherapy; carcinoma, papillary; animal experiment; animal model; body weight; xenograft model antitumor assays; cell line, tumor; protein p53; mice, inbred balb c; animalia; mus musculus; monoclonal antibody; immunoenzyme techniques; cancer inhibition; antibodies, monoclonal; xenograft; nude mouse; mice, nude; cancer size; vascular endothelial growth factor; tumor suppressor protein p53; thyroid cancer; thyroid carcinoma; thyroid neoplasms; papillary carcinoma; angiogenesis inhibitors; vasculotropin antibody; injections, intraperitoneal; papillary thyroid carcinoma; priority journal; article
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