Expression of the human cancer/testis antigen NY-SAR-35 is activated by CpG island hypomethylation Journal Article


Authors: Park, J. H.; Song, M. H.; Lee, C. H.; Lee, M. K.; Park, Y. M.; Old, L.; Lee, S. Y.
Article Title: Expression of the human cancer/testis antigen NY-SAR-35 is activated by CpG island hypomethylation
Abstract: The novel cancer/testis antigen gene, NY-SAR-35, is expressed exclusively in normal testis and in various histological types of tumor. However, the NY-SAR-35 gene expression is observed to be aberrant in several cancer cell lines and tissues. The analysis of methylation status of the NY-SAR-35 gene promoter in various cancer cell lines showed that its expression was related to methylation of the promoter region. Treatment of human cancer cell lines with the demethylating agent 5-aza-2′-deoxycytidine activated the expression of the NY-SAR-35 gene. In addition, transfection experiments on various fragments of the CpG-rich gene promoter indicate that in vitro methylation of the NY-SAR-35 gene promoter results in the loss of promoter activity. The expression of NY-SAR-35 is therefore activated by hypomethylation of the CpG island in the gene promoter. © 2011 Springer Science+Business Media B.V.
Keywords: methylation; promoter region; gene expression; antigens; cell culture; cpg islands; alkylation; in-vitro; diseases; tissue; human cancer; cells; antigen gene (ny-sar-35); cancer/testis (ct) antigen; ny-sar-35; antigen genes; cancer cell lines; gene promoter; histological type; human cancer cells; hypomethylation; promoter activities
Journal Title: Biotechnology Letters
Volume: 33
Issue: 6
ISSN: 0141-5492
Publisher: Springer  
Date Published: 2011-06-01
Start Page: 1085
End Page: 1091
Language: English
DOI: 10.1007/s10529-011-0559-y
PROVIDER: scopus
PUBMED: 21318630
DOI/URL:
Notes: --- - "Export Date: 23 June 2011" - "CODEN: BILED" - "Source: Scopus"
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  1. Lloyd J Old
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