C-di-AMP binds the ydaO riboswitch in two pseudo-symmetry-related pockets Journal Article
| Authors: | Ren, A.; Patel, D. J. |
| Article Title: | C-di-AMP binds the ydaO riboswitch in two pseudo-symmetry-related pockets |
| Abstract: | The ydaO riboswitch, involved in sporulation, osmotic stress responses and cell wall metabolism, targets the second messenger cyclic-di-AMP with subnanomolar affinity. We have solved the structure of c-di-AMP bound to the Thermoanaerobacter tengcongensis ydaO riboswitch, thereby identifying a five-helical scaffold containing a zippered-up bubble, a pseudoknot and long-range tertiary base pairs. Highlights include the identification of two c-di-AMP binding pockets on the same face of the riboswitch, related by pseudo-two-fold symmetry, with potential for cross-talk between sites mediated by adjacently positioned base-stacking alignments connecting pockets. The adenine rings of bound c-di-AMP molecules are wedged between bases and stabilized by stacking, base-sugar and sugar-sugar intermolecular hydrogen bonding interactions. The structural studies are complemented by isothermal titration calorimetry-based binding studies of mutants mediating key tertiary intermolecular contacts. The T. tengcongensis ydaO riboswitch, like its Bacillus subtilis counterpart, most likely functions through a transcription termination mechanism, with the c-di-AMP bound state representing an 'off' switch. © 2014 Nature America, Inc. All rights reserved. |
| Journal Title: | Nature Chemical Biology |
| Volume: | 10 |
| Issue: | 9 |
| ISSN: | 1552-4450 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2014-09-01 |
| Start Page: | 780 |
| End Page: | 786 |
| Language: | English |
| DOI: | 10.1038/nchembio.1606 |
| PROVIDER: | scopus |
| PUBMED: | 25086509 |
| PMCID: | PMC4217635 |
| DOI/URL: | |
| Notes: | Export Date: 2 September 2014 -- CODEN: NCBAB -- Source: Scopus |
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