Methylation of TFPI2 in stool DNA: A potential novel biomarker for the detection of colorectal cancer Journal Article
| Authors: | Glöckner, S. C.; Dhir, M.; Joo, M. Y.; McGarvey, K. E.; Van Neste, L.; Louwagie, J.; Chan, T. A.; Kleeberger, W.; De Bruïne, A. P.; Smits, K. M.; Khalid-de Bakker, C. A. J.; Jonkers, D. M. A. E.; Stockbrügger, R. W.; Meijer, G. A.; Oort, F. A.; Iacobuzio-Donahue, C.; Bierau, K.; Herman, J. G.; Baylin, S. B.; Van Engeland, M.; Schuebel, K. E.; Ahuja, N. |
| Article Title: | Methylation of TFPI2 in stool DNA: A potential novel biomarker for the detection of colorectal cancer |
| Abstract: | We have used a gene expression array-based strategy to identify the methylation of tissue factor pathway inhibitor 2 (TFPI2), a potential tumor suppressor gene, as a frequent event in human colorectal cancers (CRC). TFPI2 belongs to the recently described group of embryonic cell Polycomb group (PcG)-marked genes that may be predisposed to aberrant DNA methylation in early stages of colorectal carcinogenesis. Aberrant methylation of TFPI2 was detected in almost all CRC adenomas (97%, n = 56) and stages I to IV CRCs (99%, n = 115). We further explored the potential of TFPI2 as a biomarker for the early detection of CRC using stool DNA-based assays in patients with nonmetastatic CRC and average-risk noncancer controls who were candidates for screening. TFPI2 methylation was detected in stool DNA from stage I to III CRC patients with a sensitivity of 76% to 89% and a specificity of 79% to 93%. Detection of TFPI2 methylation in stool DNA may act as a useful adjunct to the noninvasive strategies for screening of CRCs in the future. ©2009 American Association for Cancer Research. |
| Keywords: | controlled study; major clinical study; case-control studies; advanced cancer; cancer diagnosis; colorectal cancer; gene; tumor markers, biological; hct116 cells; tumor cells, cultured; algorithms; dna methylation; colorectal neoplasms; pilot projects; early cancer; early diagnosis; colorectal adenoma; carcinoma; dna mutational analysis; ht29 cells; dna determination; feces; tfpi2 gene; caco-2 cells; early detection of cancer; glycoproteins |
| Journal Title: | Cancer Research |
| Volume: | 69 |
| Issue: | 11 |
| ISSN: | 0008-5472 |
| Publisher: | American Association for Cancer Research |
| Date Published: | 2009-06-01 |
| Start Page: | 4691 |
| End Page: | 4699 |
| Language: | English |
| DOI: | 10.1158/0008-5472.can-08-0142 |
| PUBMED: | 19435926 |
| PROVIDER: | scopus |
| PMCID: | PMC3062162 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 12" - "Export Date: 30 November 2010" - "CODEN: CNREA" - "Source: Scopus" |
