Crystal structures of two active proliferating cell nuclear antigens (PCNAs) encoded by Thermococcus kodakaraensis Journal Article
| Authors: | Ladner, J. E.; Pan, M.; Hurwitz, J.; Kelman, Z. |
| Article Title: | Crystal structures of two active proliferating cell nuclear antigens (PCNAs) encoded by Thermococcus kodakaraensis |
| Abstract: | Proliferating cell nuclear antigen (PCNA) is a ring-shaped protein that encircles duplex DNA and plays an essential role in many DNA metabolic processes in archaea and eukarya. The eukaryotic and euryarchaea genomes contain a single gene encoding for PCNA. Interestingly, the genome of the euryarchaeon Thermococcus kodakaraensis contains two PCNA-encoding genes (TK0535 and TK0582), making it unique among the euryarchaea kingdom. It is shown here that the two T. kodakaraensis PCNA proteins support processive DNA synthesis by the polymerase. Both proteins form trimeric structures with characteristics similar to those of other archaeal and eukaryal PCNA proteins. One of the notable differences between the TK0535 and TK0582 rings is that the interfaces are different, resulting in different stabilities for the two trimers. The possible implications of these observations for PCNA functions are discussed. |
| Keywords: | nonhuman; dna polymerase; dna replication; dna synthesis; protein stability; enzyme activity; cloning, molecular; amino acid sequence; molecular sequence data; species specificity; crystal structure; models, molecular; dna primers; cycline; crystallization; proliferating cell nuclear antigen; three-dimensional structure; thermococcus kodakarensis; thermococcus |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 108 |
| Issue: | 7 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 2011-02-15 |
| Start Page: | 2711 |
| End Page: | 2716 |
| Language: | English |
| DOI: | 10.1073/pnas.1019179108 |
| PUBMED: | 21270332 |
| PROVIDER: | scopus |
| PMCID: | PMC3041083 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 23 June 2011" - "CODEN: PNASA" - "Source: Scopus" |
