Oncolytic immunotherapy using recombinant vaccinia virus GLV-1h68 kills sorafenib-resistant hepatocellular carcinoma efficiently Journal Article


Authors: Ady, J. W.; Heffner, J.; Mojica, K.; Johnsen, C.; Belin, L. J.; Love, D.; Chen, C. T.; Pugalenthi, A.; Klein, E.; Chen, N. G.; Yu, Y. A.; Szalay, A. A.; Fong, Y.
Article Title: Oncolytic immunotherapy using recombinant vaccinia virus GLV-1h68 kills sorafenib-resistant hepatocellular carcinoma efficiently
Abstract: Background Sorafenib is the standard systemic therapy for unresectable or recurrent hepatocellular carcinoma (HCC) but adds minimal increase in survival. Therefore, there is a great need to develop novel therapies for advanced or recurrent HCC. One emerging field of cancer treatment involves oncolytic viruses that specifically infect, replicate within, and kill cancer cells. In this study, we examined the ability of GLV-1h68, a recombinant vaccinia virus derived from the vaccine strain that was used to eradicate smallpox, to kill sorafenib-resistant (SR) HCC cell lines. Methods Four SR HCC cell lines were generated by repeated passage in the presence of sorafenib. Median inhibitory concentration was determined for all cell lines. The infectivity, viral replication, and cytotoxicity of GLV-1h68 were assayed for both parental and SR HCC cells. Results Infectivity increased in a time and concentration-dependent manner in all cell lines. All cell lines supported efficient replication of virus. No difference between the rates of cell death between the parental and SR cell lines was observed. Conclusion Our results demonstrate that the oncolytic vaccinia virus GLV-1h68 kills both parental and SR HCC cell lines efficiently. This study indicates that patients who have failed treatment with sorafenib remain viable candidates for oncolytic therapy. © 2014 Mosby, Inc. All rights reserved.
Journal Title: Surgery
Volume: 156
Issue: 2
ISSN: 0039-6060
Publisher: Elsevier Inc.  
Date Published: 2014-08-01
Start Page: 263
End Page: 269
Language: English
DOI: 10.1016/j.surg.2014.03.031
PROVIDER: scopus
PUBMED: 24957667
PMCID: PMC4216725
DOI/URL:
Notes: Export Date: 1 August 2014 -- CODEN: SURGA -- Source: Scopus
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MSK Authors
  1. Yuman Fong
    745 Fong
  2. Elizabeth Klein
    11 Klein
  3. Chin-Tung Chen
    36 Chen
  4. Justin William Ady
    9 Ady
  5. Laurence Justin Belin
    13 Belin