The role of PTEN tumor suppressor pathway staining in carcinoma in situ of the bladder Journal Article


Authors: Sfakianos, J. P.; Gellert, L. L.; Maschino, A.; Gotto, G. T.; Kim, P. H.; Al-Ahmadie, H.; Bochner, B. H.
Article Title: The role of PTEN tumor suppressor pathway staining in carcinoma in situ of the bladder
Abstract: Objectives: The PI3k/Akt pathway has been associated with the development and progression of bladder tumors, with most studies focused on papillary or muscle-invasive tumors. We sought to characterize the expression patterns of the PI3K/Akt pathway in a large cohort of high-risk preinvasive carcinoma in situ (CIS) tumors of the bladder. Our goal was to understand whether PI3K/Akt pathway alterations associated with CIS resemble early- or late-stage bladder cancers. Material and methods: We evaluated tissue specimens from 97 patients with CIS of the bladder, of which 14 had a concomitant papillary tumor. All patients were treated with intravesical bacillus Calmette-Guerin. All specimens were evaluated for PTEN, p-AKT, and p-S6 immunoreactivity. Markers were evaluated for percentage and intensity of staining and were scored using a 0 to 3+grading system. Results: PTEN staining was noted as least intense in 67% of tumor specimens and 22% of normal urothelium. P-Akt and p-S6 had intense staining in 77% and 90% of tumor specimens vs. 44% and 68% in normal tissue, respectively. Low-intensity staining for PTEN at 12 months correlated with higher recurrence risk (P = 0.026). Conclusion: We describe a large cohort of CIS bladder tumors with decreased staining intensity of PTEN and increased staining intensity of p-AKT and p-S6, similar to high-grade and high-stage papillary tumors. Low-intensity staining of PTEN at 12 months was associated with an increased risk of recurrence. © 2014 Elsevier Inc.
Keywords: bladder cancer; carcinoma in situ; pten
Journal Title: Urologic Oncology: Seminars and Original Investigations
Volume: 32
Issue: 5
ISSN: 1078-1439
Publisher: Elsevier Inc.  
Date Published: 2014-07-01
Start Page: 657
End Page: 662
Language: English
DOI: 10.1016/j.urolonc.2014.02.003
PROVIDER: scopus
PMCID: PMC4062573
PUBMED: 24840867
DOI/URL:
Notes: Urol. Oncol. Semin. Orig. Invest. -- Export Date: 8 July 2014 -- CODEN: UOSOA -- Source: Scopus
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MSK Authors
  1. Lan Lin Gellert
    11 Gellert
  2. Geoffrey Thomas Gotto
    9 Gotto
  3. Bernard Bochner
    328 Bochner
  4. Philip Hyunwoo Kim
    39 Kim