Structure-Based Enzyme Engineering and Its Impact on In Vitro Glycorandomization Journal Article
| Authors: | Thorson, J. S.; Barton, W. A.; Hoffmeister, D.; Albermann, C.; Nikolov, D. B. |
| Article Title: | Structure-Based Enzyme Engineering and Its Impact on In Vitro Glycorandomization |
| Abstract: | Glycoengineering: This review highlights the application of structure-based enzyme engineering toward enhancing the promiscuity of nucleotidylytransferases (such as Ep, whose active site is shown here) and glycosyltransferases, which are essential to the in vitro glycorandomization (IVG) strategy. IVG is a versatile strategy with great potential for the generation of novel therapeutics as illustrated by the preparation of a library of diversified vancomycin analogues. |
| Keywords: | antibiotic agent; mutation; review; nonhuman; protein conformation; animals; cardiac glycoside; drug structure; in vitro study; drug synthesis; daunorubicin; glycosylation; crystal structure; models, molecular; crystallography, x-ray; vancomycin; structure analysis; macrolide; glycoproteins; enzymes; enzyme active site; chemical modification; nucleotidyltransferase; antibiotics; transferases; pseudomonas aeruginosa; natural products; glycosyltransferase; nucleotidyltransferases; pseudomonas; staurosporine; digitoxin; protein engineering; calicheamicin; glycosyltransferases; enzyme catalysis; amphotericin; enzyme engineering; humans; priority journal; glycosyl phosphate; methymycin; pikromycin |
| Journal Title: | ChemBioChem |
| Volume: | 5 |
| Issue: | 1 |
| ISSN: | 1439-4227 |
| Publisher: | Wiley V C H Verlag Gmbh |
| Date Published: | 2004-01-03 |
| Start Page: | 16 |
| End Page: | 25 |
| Language: | English |
| DOI: | 10.1002/cbic.200300620 |
| PROVIDER: | scopus |
| PUBMED: | 14695508 |
| DOI/URL: | |
| Notes: | ChemBioChem -- Cited By (since 1996):50 -- Export Date: 16 June 2014 -- CODEN: CBCHF -- Source: Scopus |
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