Adding functional entities to plasmids Journal Article


Authors: Svahn, M. G.; Lundin, K. E.; Ge, R.; Törnquist, E.; Simonson, E. O.; Oscarsson, S.; Leijon, M.; Brandén, L. J.; Smith, C. I. E.
Article Title: Adding functional entities to plasmids
Abstract: Non-viral gene therapy constitutes an alternative to the more common use of viral-mediated gene transfer. Most gene transfer methods using naked DNA are based upon non-sequence-specific interactions between the nucleic acid and cationic lipids (lipoplex) or polymers (polyplex). We have developed a technology in which functional entities hybridize in a sequence-specific manner to the nucleic acid (bioplex). This technology is still in its infancy, but has the potential to become a useful tool, since it allows the construction of highly defined complexes containing a variety of functional entities. In its present form the bioplex technology is based upon the use of peptide/nucleic acids (PNA) as anchors. Single, or multiple, functional entities are directly coupled to the anchors. By designing plasmids, or oligonucleotides, with the corresponding anchor target sequence, complexes with desired composition can easily be generated. The long-term aim is to combine functional entities in order to achieve optimal, synergistic interactions allowing enhanced gene transfer in vivo. Copyright © 2004 John Wiley & Sons, Ltd.
Keywords: signal transduction; review; protein localization; gene targeting; protein binding; in vivo study; protein interaction; physiology; gene transfer; gene vector; genetic vectors; chemistry; genetic transfection; signal peptide; plasmid; gene therapy; plasmids; binding site; cell nucleus; oligonucleotide; polymer; oligonucleotides; virus vector; nonviral gene delivery system; nonviral gene therapy; gene transfer techniques; dna fragment; nucleic acid; peptide derivative; dna hybridization; protein sorting signals; naked dna; priority journal; peptide nucleic acid; peptide nucleic acids; karyopherin; lipoplex; non-viral vectors; peptide-nucleic acid fusions; polyplex
Journal Title: Journal of Gene Medicine
Volume: 6
Issue: Suppl. 1
ISSN: 1099-498X
Publisher: John Wiley & Sons  
Date Published: 2004-02-01
Start Page: S36
End Page: S44
Language: English
DOI: 10.1002/jgm.510
PROVIDER: scopus
PUBMED: 14978749
DOI/URL:
Notes: J. Gene Med. -- Cited By (since 1996):14 -- Export Date: 16 June 2014 -- CODEN: JGMEF -- Source: Scopus
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