Role of reporter gene imaging in molecular and cellular biology Journal Article

  


Authors: Mayer-Kuckuk, P.; Menon, L. G.; Blasberg, R. G.; Bertino, J. R.; Banerjee, D.
Article Title: Role of reporter gene imaging in molecular and cellular biology
Abstract: Molecular imaging, including reporter gene methods, provides a unique opportunity to study biology in a living subject, thereby allowing physiological events to be monitored in an intact microenvironment. This review takes a molecular and cell biology perspective on recent studies which utilize reporter gene imaging as a tool to non-invasively monitor specific molecular biology pathways in vivo. Studies in rodent models demonstrate the feasibility of reporter gene imaging to visualize and measure key cellular pathways, such as transcription, translation and protein-protein interactions. The review indicates that molecular imaging is likely to be useful in the translation of molecular biology to medicine and biotechnological applicationss. Copyright © by Walter de Gruyter.
Keywords: osteosarcoma; signal transduction; promoter region; review; nonhuman; methotrexate; positron emission tomography; glioma; monitoring; proteins; cytology; animals; mice; apoptosis; gene expression; protein degradation; protein protein interaction; green fluorescent protein; luciferase; genetic transcription; in vivo study; cancer cell culture; molecular imaging; biotechnology; protein p53; carmustine; rna; gene expression regulation; gene activation; feasibility study; protein processing; iodine 131; luminescent measurements; colon cancer; rat; positron-emission tomography; transcription; reporter gene; rna translation; translation; liver cancer; fialuridine; iodine 124; thymidine kinase; genes, reporter; metastasis potential; microenvironment; rodentia; molecular biology; dihydrofolate reductase; fluorine 18; single photon emission computer tomography; protein p21; fluorescence analysis; herpes simplex virus; optics; cell activity; non invasive measurement; bioluminescence; transcription factor nfat; guanine derivative; leukemia cell line; experimental model; carbon 11; trimetrexate; translation regulation; trans-activation (genetics); iodine 123; reporter genes; human; priority journal; multimodality imaging
Journal Title: Biological Chemistry
Volume: 385
Issue: 5
ISSN: 1431-6730
Publisher: Walter de Gruyter GmbH  
Date Published: 2004-05-01
Start Page: 353
End Page: 361
Language: English
DOI: 10.1515/bc.2004.039
PROVIDER: scopus
PUBMED: 15195994
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-2942741151&partnerID=40&md5=6d8a3ad272fb173c364aef6545719a0a
Notes: Biol. Chem. -- Cited By (since 1996):14 -- Export Date: 16 June 2014 -- CODEN: BICHF -- Source: Scopus
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MSK Authors
  1. Ronald G Blasberg
    272 Blasberg
  2. Philipp Mayer-Kuckuk
    18 Mayer-Kuckuk
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