Mouse strains with an active H2-Ea meiotic recombination hot spot exhibit increased levels of H2-Ea-specific DNA breaks in testicular germ cells Journal Article
| Authors: | Qin, J.; Richardson, L. L.; Jasin, M.; Handel, M. A.; Arnheim, N. |
| Article Title: | Mouse strains with an active H2-Ea meiotic recombination hot spot exhibit increased levels of H2-Ea-specific DNA breaks in testicular germ cells |
| Abstract: | We devised a sensitive method for the site-specific detection of rare meiotic DNA strand breaks in germ cell-enriched testicular cell populations from mice that possess or lack an active recombination hot spot at the H2-Ea gene. Using germ cells from adult animals, we found an excellent correlation between the frequency of DNA breaks in the 418-bp H2-Ea hot spot and crossover activity. The temporal appearance of DNA breaks was also studied in 7- to 18-day-old mice with an active hot spot during the first waves of spermatogenesis. The number of DNA breaks detected rose as leptotene and zygotene spermatocytes populate the testis with a peak at day 14 postpartum, when leptotene, zygotene, and early pachytene spermatocytes are the most common meiotic prophase I cell types. The number of DNA breaks drops precipitously 1 day later, when middle to late pachytene spermatocytes become the dominant subtype. The recombination-related breaks in the hot spot likely reflect SPO11-induced double-strand breaks and/or recombination intermediates containing free 3′ hydroxyl groups. |
| Keywords: | controlled study; genetics; nonhuman; molecular genetics; animal cell; mouse; spermatocyte; animal; cytology; meiosis; metabolism; animals; mice; dna damage; gene; germ cell; haplotypes; cell population; heterozygote; animalia; dna strand breakage; haplotype; germ cells; double stranded dna; genetic recombination; molecular sequence data; recombination, genetic; nucleotide sequence; base sequence; mouse strain; testis; prophase; crossing over; spermatogenesis; hydroxyl group; h-2 antigens; male; female; priority journal; article; h2 antigen; h2 ea gene |
| Journal Title: | Molecular and Cellular Biology |
| Volume: | 24 |
| Issue: | 4 |
| ISSN: | 0270-7306 |
| Publisher: | American Society for Microbiology |
| Date Published: | 2004-02-01 |
| Start Page: | 1655 |
| End Page: | 1666 |
| Language: | English |
| DOI: | 10.1128/mcb.24.4.1655-1666.2004 |
| PROVIDER: | scopus |
| PMCID: | PMC344187 |
| PUBMED: | 14749381 |
| DOI/URL: | |
| Notes: | Mol. Cell. Biol. -- Cited By (since 1996):24 -- Export Date: 16 June 2014 -- CODEN: MCEBD -- Molecular Sequence Numbers: GENBANK: V00834; -- Source: Scopus |
