Feasibility of 99mTc-annexin V for repetitive detection of apoptotic tumor response to chemotherapy: An experimental study using a rat tumor model Journal Article
| Authors: | Kuge, Y.; Sato, M.; Zhao, S.; Takei, T.; Nakada, K.; Seki, K. I.; Strauss, H. W.; Blankenberg, F. G.; Tait, J. F.; Tamaki, N. |
| Article Title: | Feasibility of 99mTc-annexin V for repetitive detection of apoptotic tumor response to chemotherapy: An experimental study using a rat tumor model |
| Abstract: | Annexin V (annexin A5), a human protein with a high affinity for phosphatidylserine, labeled with 99mTc can detect apoptosis in vivo. In the repetitive detection of apoptosis with 99mTc-annexin V, however, the specific binding of annexin V to phosphatidylserine might affect the subsequent detection of apoptosis with this compound. To determine whether there is interference with repetitive doses of annexin V, we evaluated the effects of previous administration of cold annexin V on accumulation of 99mTc-annexin V in tumors in an experimental tumor model. Methods: Rats bearing hepatoma received cyclophosphamide (150 mg/kg, intraperitoneally) 11 d after the tumor inoculation. Cold annexin V (20 μg/kg, intravenously) was administered 24 h before or after the cyclophosphamide treatment (n = 7/group). 99mTc-Annexin V was injected intravenously (radioactive dose, 5-23 MBq/kg; mass dose, 20 μg/kg), and radioactivity in tissues was determined 6 h later. Results: Accumulation of 99mT-cannexin V in tumors was not significantly affected by previous treatment with cold annexin V before or after chemotherapy. Conclusion: These results demonstrate the feasibility of 99mT-cannexin V imaging for repetitive detection of apoptosis, which is highly required in the clinical setting. |
| Keywords: | cancer chemotherapy; controlled study; unclassified drug; liver cell carcinoma; nonhuman; chemotherapy; animal; animals; animal tissue; apoptosis; animal experiment; animal model; alkylating agent; cyclophosphamide; time; time factors; feasibility study; diagnostic agent; drug distribution; isotope labeling; tissue distribution; feasibility studies; liver tumor; rat; antineoplastic agents, alkylating; radioactivity; radiopharmaceutical agent; scintiscanning; drug tissue level; rats; tumor; rats, wistar; lipocortin 5; organotechnetium compounds; annexin a5; 99mtc-annexin v; liver neoplasms, experimental; lipocortin 5 tc 99m; technetium complex; male; priority journal; article; technetium tc 99m annexin v; wistar rat |
| Journal Title: | Journal of Nuclear Medicine |
| Volume: | 45 |
| Issue: | 2 |
| ISSN: | 0161-5505 |
| Publisher: | Society of Nuclear Medicine |
| Date Published: | 2004-02-01 |
| Start Page: | 309 |
| End Page: | 312 |
| Language: | English |
| PROVIDER: | scopus |
| PUBMED: | 14960654 |
| DOI/URL: | |
| Notes: | J. Nucl. Med. -- Cited By (since 1996):27 -- Export Date: 16 June 2014 -- CODEN: JNMEA C2 - 14960654 -- Source: Scopus |
