Carbohydrates induce mono-ubiquitination of H2B in yeast Journal Article
| Authors: | Dong, L.; Xu, C. W. |
| Article Title: | Carbohydrates induce mono-ubiquitination of H2B in yeast |
| Abstract: | Histone modifications have emerged to be a major regulatory mechanism for gene expression (1-4). However, it is not clear how histone modifications are physiologically regulated. Here, we show that mono-ubiquitinated H2B at lysine 123 (uH2B) in the yeast (Saccharomyces cerevisiae) is present in exponential phase and absent in stationary phase. A wide array of carbohydrates or sugars, including glucose, fructose, mannose, and sucrose, are capable of inducing uH2B in stationary phase yeast. In contrast, non-metabolic glucose analogs are defective in inducing uH2B. Furthermore, uH2B induction is inhibited by iodoacetate, an inhibitor of glyceraldehyde-3-phosphate dehydrogenase in glycolysis. Moreover, uH2B induction is markedly impaired in yeast mutants, in which glycolytic genes are deleted. These data indicate that glycolysis is required for the carbohydrate-induced mono-ubiquitination of H2B at lysine 123. Therefore, our study reveals a novel paradigm of metabolic regulation of histone modifications. |
| Keywords: | gene deletion; nonhuman; ubiquitin; metabolism; enzyme inhibition; physiology; gene expression regulation; regulatory mechanism; ubiquitination; genetic engineering; saccharomyces cerevisiae; glucose; glyceraldehyde 3 phosphate dehydrogenase; gene induction; yeast; saccharomyces cerevisiae proteins; histone h2b; histones; glycolysis; mutagenesis; lysine; carbohydrate; chemical modification; histone modifications; saccharomyces; mannose; fructose; priority journal; article; iodoacetic acid |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 279 |
| Issue: | 3 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 2004-01-16 |
| Start Page: | 1577 |
| End Page: | 1580 |
| Language: | English |
| DOI: | 10.1074/jbc.C300505200 |
| PROVIDER: | scopus |
| PUBMED: | 14660635 |
| DOI/URL: | |
| Notes: | J. Biol. Chem. -- Cited By (since 1996):14 -- Export Date: 16 June 2014 -- CODEN: JBCHA -- Source: Scopus |
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