Ablation of PARP-1 does not interfere with the repair of DNA double-strand breaks, but compromises the reactivation of stalled replication forks Journal Article
| Authors: | Yang, Y. G.; Cortes, U.; Patnaik, S.; Jasin, M.; Wang, Z. Q. |
| Article Title: | Ablation of PARP-1 does not interfere with the repair of DNA double-strand breaks, but compromises the reactivation of stalled replication forks |
| Abstract: | Poly(ADP-ribose) polymerase-1 (PARP-1) is an abundant DNA end-sensing and binding molecule. Inactivation of PARP-1 by chemicals and genetic imitations slows cell proliferation, increases sister chromatid exchange (SCE), micronuclei formation and chromosome instability, and shortens telomeres. Given its affinity to DNA breaks and temporal occupation on DNA strand break sites, PARP-1 is proposed to prevent inappropriate DNA recombination. We investigated the potential role of PARP-1 in repair of DNA double-strand breaks (DSBs) and stalled replication forks. PARP-1-/- embryonic stem cells and embryonic fibroblast cells exhibited normal repair of DNA DSBs by either homologous recombination (HR) or nonhomologous end-joining (NHEJ) pathways. Inactivation of PARP-1 did not interfere with gene-targeting efficiency in ES cells. However, PARP-1-/- cells were hypersensitive to the replication damage agent hydroxyurea (HU)-induced cell death and exhibited enhanced SCE formation. Ablation of PARP-1 delayed reactivation of stalled replication forks imposed by HU and re-entry into the G2-M phase after HU release. These data indicate that PARP-1 is dispensable in HR induced by DSBs, but is involved in the repair and reactivation of stalled replication forks. |
| Keywords: | controlled study; dna binding protein; dna-binding proteins; hydroxyurea; nonhuman; dna replication; animal cell; mouse; animal; animals; mice; cell death; dna damage; homologous recombination; gene targeting; dna repair; embryo; gene function; physiology; animalia; stem cell; dna strand breakage; double stranded dna; genetic recombination; recombination, genetic; enzyme inactivation; fibroblast; dna double-strand break repair; sister chromatid exchange; cell cycle g2 phase; nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase; rad51 protein; rad51 recombinase; rad51 protein, mouse; poly(adp-ribose) polymerases; poly(adp-ribose) polymerase-1; rad51; priority journal; article; replication stall and progression |
| Journal Title: | Oncogene |
| Volume: | 23 |
| Issue: | 21 |
| ISSN: | 0950-9232 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2004-03-15 |
| Start Page: | 3872 |
| End Page: | 3882 |
| Language: | English |
| DOI: | 10.1038/sj.onc.1207491 |
| PROVIDER: | scopus |
| PUBMED: | 15021907 |
| DOI/URL: | |
| Notes: | Oncogene -- Cited By (since 1996):100 -- Export Date: 16 June 2014 -- CODEN: ONCNE -- Source: Scopus |
