Interstitial infusion of IL13-PE38QQR in the rat brain stem Journal Article
| Authors: | Souweidane, M. M.; Occhiogrosso, G.; Mark, E. B.; Edgar, M. A. |
| Article Title: | Interstitial infusion of IL13-PE38QQR in the rat brain stem |
| Abstract: | Interstitial infusion of IL13-PE38QQR, a tumor specific, chimeric cytotoxin, into the rat brain stem was performed in an effort to assess safety. Six rats underwent stereotaxic cannula placement into the pontine segment of the brain stem followed by a 24-h infusion of IL13-PE38QQR (volume of infusion (Vi) 200 μl) at a concentration of 10 μg/ml. The animals were assessed neurologically and then sacrificed either immediately or after 2 weeks. All animals tolerated the infusions without exhibiting any neurological changes. Postmortem examination of the brains revealed no significant histological changes beyond the site of the cannula tract. These findings indicate that supratherapeutic concentrations of IL13-PE38QQR administered by interstitial infusion into the rat brain stem is well tolerated and may serve as a potential therapeutic strategy for children with diffuse pontine gliomas. © 2004 Kluwer Academic Publishers. |
| Keywords: | controlled study; genetics; drug tolerability; histopathology; drug safety; nonhuman; glioma; animal; metabolism; animals; animal tissue; interleukin 13; animal experiment; animal model; drug effect; pathology; drug specificity; time; chimera; hybrid protein; recombinant fusion proteins; rat; drug infusion; toxicity testing; rats; rats, sprague-dawley; autopsy; pons angle tumor; nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase; brain stem; infusion; infusions, parenteral; sprague dawley rat; concentration response; interstitial infusion; neurologic examination; drug indication; stereotaxic surgery; interleukin-13; recombinant interleukin 13; cytotoxin; brain stem glioma; infusion pump; immunotoxin; immunotoxins; stereotaxic techniques; convection-enhanced delivery; pons; cannulation; il13-pe38qqr; exotoxin; adp ribose transferases; bacterial toxins; exotoxins; virulence factors; virulence factor; pontine glioma; infusion pumps; bacterial toxin; male; article; interstitium; il-13 cytotoxin; il13 pe38qqr; toxa protein, pseudomonas aeruginosa |
| Journal Title: | Journal of Neuro-Oncology |
| Volume: | 67 |
| Issue: | 3 |
| ISSN: | 0167-594X |
| Publisher: | Springer |
| Date Published: | 2004-05-01 |
| Start Page: | 287 |
| End Page: | 293 |
| Language: | English |
| DOI: | 10.1023/b:neon.0000024219.47447.91 |
| PROVIDER: | scopus |
| PUBMED: | 15164984 |
| DOI/URL: | |
| Notes: | J. Neuro-Oncol. -- Cited By (since 1996):12 -- Export Date: 16 June 2014 -- CODEN: JNODD -- Source: Scopus |
