Specific recognition of rpsO mRNA and 16S rRNA by Escherichia coli ribosomal protein S15 relies on both mimicry and site differentiation Journal Article
| Authors: | Mathy, N.; Pellegrini, O.; Serganov, A.; Patel, D. J.; Ehresmann, C.; Portier, C. |
| Article Title: | Specific recognition of rpsO mRNA and 16S rRNA by Escherichia coli ribosomal protein S15 relies on both mimicry and site differentiation |
| Abstract: | The ribosomal protein S15 binds to 16S rRNA, during ribosome assembly, and to its own mRNA (rpsO mRNA), affecting autocontrol of its expression. In both cases, the RNA binding site is bipartite with a common subsite consisting of a G•U/G-C motif. The second subsite is located in a three-way junction in 16S rRNA and in the distal part of a stem forming a pseudoknot in Escherichia coli rpsO mRNA. To determine the extent of mimicry between these two RNA targets, we determined which amino acids interact with rpsO mRNA. A plasmid carrying rpsO (the S15 gene) was mutagenized and introduced into a strain lacking S15 and harbouring an rpsO-lacZ translational fusion. Analysis of deregulated mutants shows that each subsite of rpsO mRNA is recognized by a set of amino acids known to interact with 16S rRNA. In addition to the G•U/G-C motif, which is recognized by the same amino acids in both targets, the other subsite interacts with amino acids also involved In contacts with helix H22 of 16S rRNA, in the region adjacent to the three-way junction. However, specific S15-rpsO mRNA interactions can also be found, probably with A(-46) in loop L1 of the pseudoknot, demonstrating that mimicry between the two targets is limited. |
| Keywords: | controlled study; unclassified drug; nonhuman; binding affinity; protein motif; gene expression; amino acid sequence; molecular sequence data; recombinant fusion proteins; messenger rna; rna, messenger; sequence alignment; nucleotide sequence; escherichia coli; gene fusion; beta galactosidase; rna translation; base sequence; binding site; models, molecular; mutagenesis, site-directed; molecular interaction; nucleic acid conformation; rna 16s; site directed mutagenesis; protein structure, secondary; rna binding; ribosomal proteins; ribosome protein; escherichia coli proteins; gene expression regulation, bacterial; protein rna binding; prokaryota; molecular mimicry; negibacteria; protein s15; rna, ribosomal, 16s; priority journal; article |
| Journal Title: | Molecular Microbiology |
| Volume: | 52 |
| Issue: | 3 |
| ISSN: | 0950-382X |
| Publisher: | Blackwell Publishing |
| Date Published: | 2004-05-01 |
| Start Page: | 661 |
| End Page: | 675 |
| Language: | English |
| DOI: | 10.1111/j.1365-2958.2004.04005.x |
| PROVIDER: | scopus |
| PUBMED: | 15101974 |
| PMCID: | PMC4693643 |
| DOI/URL: | |
| Notes: | Mol. Microbiol. -- Cited By (since 1996):23 -- Export Date: 16 June 2014 -- CODEN: MOMIE -- Molecular Sequence Numbers: GENBANK: U13872; -- Source: Scopus |
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