ATR, BRCA1 and γH2AX localize to unsynapsed chromosomes at the pachytene stage in human oocytes Journal Article
| Authors: | Garcia-Cruz, R.; Roig, I.; Robles, P.; Scherthan, H.; Caldés, M. G. |
| Article Title: | ATR, BRCA1 and γH2AX localize to unsynapsed chromosomes at the pachytene stage in human oocytes |
| Abstract: | Asynapsis of homologous chromosomes at the pachytene stage has been associated with gametogenic failure and infertility, but the cellular mechanisms involved are currently unknown in human meiocytes. In mice, the protein encoded by the breast-cancer susceptibility gene Brca1 has been described to direct kinase ATR (ataxia telangiectasia and Rad3 related) to any unpaired DNA at the pachytene stage, where ATR triggers H2AX phosphorylation, resulting in the silencing of those chromosomes. In this study, the distribution of ATR, BRCA1 and the phosphorylated histone γH2AX is assessed by immunofluorescence in human oocytes and it is found that they localize at unpaired chromosomes at the pachytene stage. Evidence is shown to propose that BRCA1, ATR and γH2AX in the human maybe part of a system such as the one previously described in mouse, which signals unsynapsed chromosomes at pachytene and may lead to their silencing. © 2009 Published by Reproductive Healthcare Ltd. |
| Keywords: | signal transduction; controlled study; human tissue; protein phosphorylation; human cell; protein localization; cell cycle protein; oocyte; pachytene; meiosis; metabolism; cell cycle proteins; oocytes; protein binding; immunofluorescence; protein serine threonine kinase; brca1 protein; physiology; tissue distribution; histone; protein-serine-threonine kinases; fetus; gene silencing; chromosome analysis; chromosomes, human; chromosome pairing; human chromosome; γh2ax; asynapsis; atr; brca1; atr protein; histone h2ax; rna polymerase ii; atr protein, human; brca1 protein, human; h2afx protein, human; genome imprinting; genomic imprinting; histones; pachytene stage |
| Journal Title: | Reproductive BioMedicine Online |
| Volume: | 18 |
| Issue: | 1 |
| ISSN: | 1472-6483 |
| Publisher: | Reproductive Healthcare Ltd |
| Date Published: | 2009-01-01 |
| Start Page: | 37 |
| End Page: | 44 |
| Language: | English |
| PUBMED: | 19146767 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 5" - "Export Date: 30 November 2010" - "CODEN: RBOEA" - "Source: Scopus" |
