Hypoxia markers in human osteosarcomas Journal Article

  


Authors: García-Castellano, J. M.; Gorlick, R.
Article Title: Hypoxia markers in human osteosarcomas
Abstract: The aim of this work was to investigate the frequency of HIF-1α and VEGF expression, the neovascularization and cell proliferation in human osteosarcoma. Expression of HIF-1α and VEGF were assessed by immunohistochemistry. Neovascularization was evaluated by immunostaining against factor VIII, and cell proliferation was determined by immunohistochemistry against Ki-67. Statistical association between these parameters and clinical features was examined. HIF-1α staining was positive in 35% of patients, being clinically correlated with metastasis. VEGF expression was detected in 69% of cases, and was significantly associated with gender. VEGF was also associated with histological tumor grade. No association was found between cell proliferation and clinical data. In conclusion, this manuscript shows the first description of HIF-1α expression in osteosarcomas, which was associated with the metastatic disease; it is also shown an association between VEGF protein expression with female gender; and a trend towards an association between VEGF expression and tumor grade.
Keywords: immunohistochemistry; osteosarcoma; vasculotropin; human tissue; protein expression; major clinical study; clinical feature; cancer grading; ki 67 antigen; cell proliferation; biological marker; metastasis; histology; hypoxia; gender; hypoxia inducible factor 1alpha; neovascularization (pathology); vegf; blood clotting factor 8; factor viii; ki 67; hif-1α; correlation function; human; male; female; article
Journal Title: Mapfre Medicina
Volume: 15
Issue: 1
ISSN: 1130-5665
Publisher: Fundación MAPFRE  
Date Published: 2004-01-01
Start Page: 3
End Page: 10
Language: Spanish
PROVIDER: scopus
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-1942508079&partnerID=40&md5=59c06c01d1afa75fcb75c7ad0cb2e64a
Notes: Mapfre Med. -- Cited By (since 1996):2 -- Export Date: 16 June 2014 -- CODEN: MAMEE -- Source: Scopus