Liver sinusoidal endothelial cells are insufficient to activate T cells Journal Article
| Authors: | Katz, S. C.; Pillarisetty, V. G.; Bleier, J. I.; Shah, A. B.; DeMatteo, R. P. |
| Article Title: | Liver sinusoidal endothelial cells are insufficient to activate T cells |
| Abstract: | Liver sinusoidal endothelial cells (LSEC) have been reported to express MHC class II, CD80, CD86, and CD11c and effectively stimulate naive T cells. Because dendritic cells (DC) are known to possess these characteristics, we sought to directly compare the phenotype and function of murine LSEC and DC. Nonparenchymal cells from C57BL/6 mice were obtained by collagenase digestion of the liver followed by density gradient centrifugation. From the enriched nonparenchymal cell fraction, LSEC (CD45-) were then isolated to 99% purity using immunomagnetic beads. Flow cytometric analysis of LSEC demonstrated high expression of CD31, von Willebrand factor, and FcyRs. However, unlike DC, LSEC had low or absent expression of MHC class II, CD86, and CD11c. LSEC demonstrated a high capacity for Ag uptake in vitro and in vivo. Although acetylated low-density lipoprotein uptake has been purported to be a specific function of LSEC, we found DC captured acetylated low-density lipoprotein to a similar extent in vivo. Consistent with their phenotype, LSEC were poor stimulators of allogeneic T cells. Furthermore, in the absence of exogenous costimulation, LSEC induced negligible proliferation of CD4+ or CD8+ TCR-transgenic T cells. Thus, contrary to previous reports, our data indicate that LSEC alone are insufficient to activate naive T cells. |
| Keywords: | adolescent; controlled study; nonhuman; flow cytometry; antigen expression; lymphocyte proliferation; t-lymphocytes; animal cell; mouse; phenotype; animals; mice; cell function; dendritic cell; in vivo study; in vitro study; mice, inbred balb c; mice, inbred c57bl; transgenic mouse; endothelium cell; endothelial cells; t lymphocyte receptor; liver; lymphocyte activation; dendritic cells; antigen; immunomagnetic separation; major histocompatibility antigen class 2; fc receptor; cell fractionation; immunostimulation; mouse strain; histocompatibility antigens class ii; collagenase; t lymphocyte activation; cd45 antigen; b7 antigen; cd86 antigen; cd31 antigen; alloimmunity; costimulation; antigens, cd45; glycoprotein p 15095; density gradient centrifugation; von willebrand factor; male; priority journal; article; acetyl low density lipoprotein; liver sinusoid; liver sinusoid endothelium cell |
| Journal Title: | Journal of Immunology |
| Volume: | 173 |
| Issue: | 1 |
| ISSN: | 0022-1767 |
| Publisher: | The American Association of Immunologists, Inc |
| Date Published: | 2004-07-01 |
| Start Page: | 230 |
| End Page: | 235 |
| Language: | English |
| PROVIDER: | scopus |
| PUBMED: | 15210779 |
| DOI: | 10.4049/jimmunol.173.1.230 |
| DOI/URL: | |
| Notes: | J. Immunol. -- Cited By (since 1996):63 -- Export Date: 16 June 2014 -- CODEN: JOIMA C2 - 15210779 -- Source: Scopus |
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637DeMatteo -
29Pillarisetty -
33
Katz -
13
Bleier -
20
Shah
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