B cell signaling is regulated by induced palmitoylation of CD81 Journal Article
| Authors: | Cherukuri, A.; Carter, R. H.; Brooks, S.; Bornmann, W.; Finn, R.; Dowd, C. S.; Pierce, S. K. |
| Article Title: | B cell signaling is regulated by induced palmitoylation of CD81 |
| Abstract: | Signaling through the B cell antigen receptor (BCR) is amplified and prolonged by coligation of the BCR to the CD19/CD21/CD81 coreceptor complex. Coligation is induced during immune responses by the simultaneous binding of complement-tagged antigens to the complement receptor, CD21, and to the BCR. Enhanced signaling is due in part to the ability of the CD19/CD21/CD81 complex to stabilize the BCR in sphingolipid- and cholesterol-rich membrane microdomains termed lipid rafts. The tetraspanin CD81 is essential for the raft-stabilizing function of the coreceptor. Here we show that coligation of the BCR and the CD19/CD21/CD81 complex leads to selective, rapid, and reversible palmitoylation of CD81 and that palmitoylation is necessary for the raft stabilizing function of the CD19/CD21/CD81 complex. Inducible palmitoylation may represent a novel mechanism by which tetraspanins function to facilitate lipid raft-dependent receptor signaling. |
| Keywords: | signal transduction; controlled study; human cell; protein function; complex formation; cell line; b lymphocyte; b-lymphocytes; immunology; antibodies, monoclonal; antigens; cholesterol; lipids; antigens, cd; antigen binding; cd19 antigen; antigens, cd19; macromolecular substances; cells; cell signaling; palmitoylation; receptors, antigen, b-cell; cross-linking reagents; tetraspanin; complement component c3d receptor; receptors, complement 3d; lipid membrane; membrane microdomains; b lymphocyte receptor; palmitic acid; sphingolipid; cd81 antigen; receptor intrinsic activity; humans; human; priority journal; article; b cell antigen receptor (bcr); coligation |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 279 |
| Issue: | 30 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 2004-07-23 |
| Start Page: | 31973 |
| End Page: | 31982 |
| Language: | English |
| DOI: | 10.1074/jbc.M404410200 |
| PROVIDER: | scopus |
| PUBMED: | 15161911 |
| DOI/URL: | |
| Notes: | J. Biol. Chem. -- Cited By (since 1996):67 -- Export Date: 16 June 2014 -- CODEN: JBCHA -- Source: Scopus |
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