Constitutive activation of STAT5A promotes human hematopoietic stem cell self-renewal and erythroid differentiation Journal Article

   

Authors:	Schuringa, J. J.; Chung, K. Y.; Morrone, G.; Moore, M. A. S.
Article Title:	Constitutive activation of STAT5A promotes human hematopoietic stem cell self-renewal and erythroid differentiation
Abstract:	Activation of the transcription factor signal transducer and activator of transcription (STAT)5 is involved in various aspects of hematopoiesis, affecting cell proliferation, differentiation, and cell survival. Constitutive activation of STAT5 has also been associated with leukemic transformation. We overexpressed the constitutively active mutant STAT5A(1*6) in human cord blood CD34+ cells and evaluated the effects on the hematopoietic potential of stem cells in a variety of in vitro and in vivo systems. The observed phenotypic changes were correlated with differential gene expression patterns induced by STAT5A(1*6). Our data indicate that a persistent activation of STAT5A in human hematopoietic stem and progenitor cells results in their enhanced self-renewal and diverts differentiation to the erythroid lineage.
Keywords:	signal transduction; controlled study; leukemia; human cell; mutation; dna-binding proteins; flow cytometry; polymerase chain reaction; cell proliferation; phenotype; animals; mice; cell survival; cell division; gene expression; erythropoiesis; cell line; cell renewal; cell differentiation; mice, scid; time factors; luminescent proteins; cell transformation, neoplastic; correlation analysis; fetal blood; reverse transcriptase polymerase chain reaction; oligonucleotide array sequence analysis; nucleotide sequence; erythroid cell; hematopoietic stem cells; immunoblotting; stem cells; umbilical cord blood; green fluorescent proteins; hematopoiesis; trans-activators; hematopoietic stem cell; mice, inbred nod; stat5 transcription factor; antigens, cd34; retroviridae; erythrocytes; cd34; stat5a protein; milk proteins; humans; human; priority journal; article; stat5a(1*6)
Journal Title:	Journal of Experimental Medicine
Volume:	200
Issue:	5
ISSN:	0022-1007
Publisher:	Rockefeller University Press
Date Published:	2004-09-06
Start Page:	623
End Page:	635
Language:	English
DOI:	10.1084/jem.20041024
PROVIDER:	scopus
PMCID:	PMC2212750
PUBMED:	15353555
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-4544371118&partnerID=40&md5=80d61c3423b070090383278a9786587a
Notes:	J. Exp. Med. -- Cited By (since 1996):80 -- Export Date: 16 June 2014 -- CODEN: JEMEA -- Molecular Sequence Numbers: GENBANK: HS100764, HS108694, HS117848, HS153261, HS155024, HS161002, HS169536, HS169610, HS1817, HS183109, HS183805, HS2250, HS226627, HS234642, HS2490, HS266959, HS272003, HS272572, HS278426, HS301612, HS303157, HS326392, HS343871, HS347939, HS8257, HS8375, HS9963; -- Source: Scopus

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