BRCA1-mediated repression of select X chromosome genes Journal Article
| Authors: | Jazaeri, A. A.; Chandramouli, G. V. R.; Aprelikova, O.; Nuber, U. A.; Sotiriou, C.; Liu, E. T.; Ropers, H. H.; Yee, C. J.; Boyd, J.; Barrett, J. C. |
| Article Title: | BRCA1-mediated repression of select X chromosome genes |
| Abstract: | Recently BRCA1 has been implicated in the regulation of gene expression from the X chromosome. In this study the influence of BRCA1 on expression of X chromosome genes was investigated. Complementary DNA microarrays were used to compare the expression levels of X chromosome genes in 18 BRCA1-associated ovarian cancers to those of the 13 "BRCA1-like" and 14 "BRCA2-like" sporadic tumors (as defined by previously reported expression profiling). Significance was determined using parametric statistics with P < 0.005 as a cutoff. Forty of 178 total X-chromosome transcripts were differentially expressed between the BRCA1-associated tumors and sporadic cancers with a BRCA2-like molecular profile. Thirty of these 40 genes showed higher mean expression in the BRCA1-associated samples including all 11 transcripts that mapped to Xp11. In contrast, four of 178 total X chromosome transcripts showed significant differential expression between BRCA1-associated and sporadic tumors with a BRCA1-like molecular profile. All four mapped to Xp11 and showed higher mean expression in BRCA1-associated tumors. Reexpression of BRCA1 in HCC1937 BRCA1-deficient breast cancer cell resulted in the repression of 21 transcripts. Eleven of the 21 (54.5%) transcripts mapped to Xp11. However, there was no significant overlap between these Xp11 genes and those found to be differentially expressed between BRCA1-associated and sporadic ovarian cancer samples. These results demonstrate that BRCA1 mediates the repression of several X chromosome genes, many of which map to the Xp11 locus. © 2004 Jazaeri et al; licensee BioMed Central Ltd. |
| Keywords: | controlled study; human cell; genetic analysis; ovary cancer; breast cancer; gene expression profiling; gene locus; genetic variability; genetic transcription; brca1 protein; brca2 protein; gene expression regulation; gene mapping; statistical analysis; statistical significance; cancer cell; gene repression; x chromosome; dna microarray; molecular biology; complementary dna; genetic selection; chromosome xp; human; article |
| Journal Title: | Journal of Translational Medicine |
| Volume: | 2 |
| ISSN: | 1479-5876 |
| Publisher: | Biomed Central Ltd |
| Date Published: | 2004-09-21 |
| Language: | English |
| DOI: | 10.1186/1479-5876-2-32 |
| PROVIDER: | scopus |
| PMCID: | PMC520825 |
| PUBMED: | 15383145 |
| DOI/URL: | |
| Notes: | J. Transl. Med. -- Cited By (since 1996):17 -- Export Date: 16 June 2014 -- Source: Scopus |
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