The Drosophila miR-310 cluster negatively regulates synaptic strength at the neuromuscular junction Journal Article

   

Authors:	Tsurudome, K.; Tsang, K.; Liao, E. H.; Ball, R.; Penney, J.; Yang, J. S.; Elazzouzi, F.; He, T.; Chishti, A.; Lnenicka, G.; Lai, E. C.; Haghighi, A. P.
Article Title:	The Drosophila miR-310 cluster negatively regulates synaptic strength at the neuromuscular junction
Abstract:	Emerging data implicate microRNAs (miRNAs) in the regulation of synaptic structure and function, but we know little about their role in the regulation of neurotransmission in presynaptic neurons. Here, we demonstrate that the miR-310-313 cluster is required for normal synaptic transmission at the Drosophila larval neuromuscular junction. Loss of miR-310-313 cluster leads to a significant enhancement of neurotransmitter release, which can be rescued with temporally restricted expression of mir-310-313 in larval presynaptic neurons. Kinesin family member, Khc-73 is a functional target for miR-310-313 as its expression is increased in mir-310-313 mutants and reducing it restores normal synaptic function. Cluster mutants show an increase in the active zone protein Bruchpilot accompanied by an increase in electron dense T bars. Finally, we show that repression of Khc-73 by miR-310-313 cluster influences the establishment of normal synaptic homeostasis. Our findings establish a role for miRNAs in the regulation of neurotransmitter release. © 2010 Elsevier Inc.
Keywords:	controlled study; protein expression; unclassified drug; gene cluster; nonhuman; mutant protein; animal cell; animals; animal tissue; microrna; drosophila; motor neurons; micrornas; homeostasis; drosophila proteins; kinesin; larva; multigene family; motoneuron; synaptic transmission; neuromuscular synapse; microrna 310; microrna 311; microrna 312; microrna 313; protein khc 73; neurotransmitter release; synaptosome; neuromuscular junction
Journal Title:	Neuron
Volume:	68
Issue:	5
ISSN:	0896-6273
Publisher:	Cell Press
Date Published:	2010-12-09
Start Page:	879
End Page:	893
Language:	English
DOI:	10.1016/j.neuron.2010.11.016
PUBMED:	21145002
PROVIDER:	scopus
PMCID:	PMC3034365
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-78649974217&partnerID=40&md5=bb0f8686a70790eca29f4d6791ecbcbd
Notes:	--- - "Export Date: 20 April 2011" - "CODEN: NERNE" - "Source: Scopus"

https://synapse.mskcc.org/synapse/works/has_related_data_chk/5260