Structure, mechanism, and specificity of a eukaryal tRNA restriction enzyme involved in self-nonself discrimination Journal Article

   

Authors:	Chakravarty, A.; Smith, P.; Jalan, R.; Shuman, S.
Article Title:	Structure, mechanism, and specificity of a eukaryal tRNA restriction enzyme involved in self-nonself discrimination
Abstract:	tRNA restriction by anticodon nucleases underlies cellular stress responses and self-nonself discrimination in a wide range of taxa. Anticodon breakage inhibits protein synthesis, which, in turn, results ingrowth arrest or cell death. The eukaryal ribotoxinPaT secreted by Pichia acaciae inhibits growthofSaccharomyces cerevisiae via cleavage of tRNAGln(UUG). We find that recombinant PaT incisesa synthetic tRNAGln(UUG) stem-loop RNA by transesterification at a single site 3' of the wobble uridine, yielding 2',3'-cyclic phosphate and 5'-OH ends. Incision is suppressed by replacement of the wobble nucleobase with adenine or guanine. The crystal structure of PaT reveals a distinctive fold and active site, essential components of which are demonstrated by mutagenesis. Pichia acaciae evades self-toxicity via a distinctive intracellular immunity protein, ImmPaT, which binds PaT and blocks nuclease activity. Our results highlight the evolutionary diversity of tRNA restriction and immunity systems. © 2014 The Authors.
Journal Title:	Cell Reports
Volume:	7
Issue:	2
ISSN:	2211-1247
Publisher:	Cell Press
Date Published:	2014-04-24
Start Page:	339
End Page:	347
Language:	English
DOI:	10.1016/j.celrep.2014.03.034
PROVIDER:	scopus
PUBMED:	24726365
PMCID:	PMC4121121
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-84899630852&partnerID=40&md5=6788331d4a3402bff0e1c5b3d4827415
Notes:	Cell Rep. -- Export Date: 2 June 2014 -- Source: Scopus

https://synapse.mskcc.org/synapse/works/has_related_data_chk/51723