Glioma oncoprotein Bc12L12 inhibits the p53 tumor suppressor Journal Article
| Authors: | Stegh, A. H.; Brennan, C.; Mahoney, J. A.; Forloney, K. L.; Jenq, H. T.; Luciano, J. P.; Protopopov, A.; Chin, L.; DePinho, R. A. |
| Article Title: | Glioma oncoprotein Bc12L12 inhibits the p53 tumor suppressor |
| Abstract: | Glioblastoma multiforme (GBM) is a lethal brain tumor characterized by intense apoptosis resistance and extensive necrosis. Bcl2L12 (for Bcl2-like 12) is a cytoplasmic and nuclear protein that is overexpressed in primary GBM and functions to inhibit post-mitochondrial apoptosis signaling. Here, we show that nuclear Bcl2L12 physically and functionally interacts with the p53 tumor suppressor, as evidenced by the capacity of Bcl2L12 to (1) enable bypass of replicative senescence without concomitant loss of p53 or p19Arf, (2) inhibit p53-dependent DNA damage-induced apoptosis, (3) impede the capacity of p53 to bind some of its target gene promoters, and (4) attenuate endogenous p53-directed transcriptomic changes following genotoxic stress. Correspondingly, The Cancer Genome Atlas profile and tissue protein analyses of human GBM specimens show significantly lower Bcl2L12 expression in the setting of genetic p53 pathway inactivation. Thus, Bcl2L12 is a multifunctional protein that contributes to intense therapeutic resistance of GBM through its ability to operate on two key nodes of cytoplasmic and nuclear signaling cascades. © 2010 by Cold Spring Harbor Laboratory Press. |
| Keywords: | signal transduction; human tissue; unclassified drug; oncoprotein; promoter region; pathophysiology; glioma; protein function; protein analysis; mouse; animal; metabolism; animals; mice; dna damage; cells, cultured; gene targeting; protein bcl 2; apoptosis; gene expression; nuclear protein; embryo; protein depletion; protein protein interaction; cell line; protein binding; protein stability; genetic transcription; protein p53; physiology; cancer resistance; gene expression regulation; cell culture; promoter regions, genetic; protein transport; tumor suppressor protein p53; stress; glioblastoma multiforme; gene inactivation; proto-oncogene proteins c-bcl-2; cell aging; p53; genotoxicity; bc12l12; bcl 2 like 12 protein; bcl2l12 protein, mouse |
| Journal Title: | Genes and Development |
| Volume: | 24 |
| Issue: | 19 |
| ISSN: | 0890-9369 |
| Publisher: | Cold Spring Harbor Laboratory Press |
| Date Published: | 2010-10-01 |
| Start Page: | 2194 |
| End Page: | 2204 |
| Language: | English |
| DOI: | 10.1101/gad.1924710 |
| PUBMED: | 20837658 |
| PROVIDER: | scopus |
| PMCID: | PMC2947771 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 3" - "Export Date: 20 April 2011" - "CODEN: GEDEE" - "Source: Scopus" |
